In the dairy industry financial losses mainly due to loss of milk production caused by mastitis and mastitis related problems is very common. In the mastitis, identification of the infectious agent/s could be of great help. In the present study, a multiplex polymerase chain reaction (PCR) to identify five common organisms implicated in the mastitis was tried. Primers for identification of Staphylococcus aureus, Escherichia coli (E. coli), Klebsiella pneumoniae, Pseudomonas aeruginosa, and Streptococcus agalactiae were selected/ designed. DNA was extracted from the affected milk using two different methods and was subjected to multiplex PCR. It was observed from the study that a multiplex PCR to amplify four organisms E. coli, K. pneumoniae, S. agalactiae and P. aeruginosa was developed successfully to quicken the detection of common mastitis causing organisms.

中文翻译：

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开发用于鉴定铜绿假单胞菌，大肠杆菌，肺炎克雷伯菌和无乳链球菌的多重PCR方法

在乳业中，经济损失主要是由于乳腺炎和与乳腺炎有关的问题而导致的牛奶生产损失非常普遍。在乳腺炎中，识别传染原可能会很有帮助。在本研究中，尝试了多重聚合酶链反应（PCR）来鉴定与乳腺炎有关的五个常见生物。鉴定金黄色葡萄球菌，大肠杆菌（E. coli），肺炎克雷伯菌，铜绿 假单胞菌和无乳链球菌的引物被选择/设计。使用两种不同方法从受影响的牛奶中提取DNA，然后进行多重PCR。从该研究中观察到，成功开发了用于扩增四种生物的大肠杆菌，肺炎克雷伯氏菌，无乳链球菌和铜绿假单胞菌的多重PCR，以加快对引起乳腺炎的常见生物的检测。