Chitosan (CS) and Platelet-Rich Plasma (PRP) both display interesting properties for wound healing applications. A hybrid CS-PRP biomaterial was previously developped, consisting of a freeze dried CS formulation solubilized in PRP that promotes tissue repair and regeneration. The purpose of the current study was to investigate the ability of the CS-PRP biomaterial to stimulate cell migration in vitro. Scratch assays revealed that CS-PRP significantly stimulates the migration rate of cells compared to cells in culture medium but not differently than PRP alone. The increase in the migration rate is dose-dependent at low dose and reaches a plateau corresponding with maximum cell motility. Cell migration rate as a function of the number of platelets that have degranulated in culture medium (to which total concentration of growth factors contributing to cell response is proportionnal), follows a modified Hill model. To analyze photographs taken during the assay and follow cell migration, an open source image analysis algorithm was developed: SAMScratch (Systematic Area Measurement of Scratch - available here: https://github.com/Biomaterials-and-Cartilage-Laboratory/SAM-Scratch). Compared with other existing analysis tools, the algorithm is precise in the determination of the scratch area and performs equally well with usual and challenging images. This study resulted in the creation of a freely available application for scratch assay analysis and provided evidence that CS-PRP implants hold promise for treatment of wounds.

壳聚糖 (CS) 和富含血小板的血浆 (PRP) 都显示出用于伤口愈合应用的有趣特性。先前开发了一种混合 CS-PRP 生物材料，由溶解在 PRP 中的冷冻干燥 CS 制剂组成，可促进组织修复和再生。本研究的目的是研究 CS-PRP 生物材料在体外刺激细胞迁移的能力. 划痕分析显示，与培养基中的细胞相比，CS-PRP 显着刺激细胞的迁移率，但与单独的 PRP 没有区别。迁移率的增加在低剂量下是剂量依赖性的，并达到与最大细胞运动相对应的平台期。细胞迁移率作为在培养基中脱颗粒的血小板数量的函数（促进细胞反应的生长因子的总浓度与其成正比），遵循改良的 Hill 模型。为了分析测定过程中拍摄的照片并跟踪细胞迁移，开发了一种开源图像分析算法：SAMScratch（划痕的系统面积测量 - 可在此处获得：https://github.com/Biomaterials-and-Cartilage-Laboratory/SAM-挠）。与现有的其他分析工具相比，该算法在确定划痕区域方面非常精确，并且在处理普通图像和具有挑战性的图像时表现同样出色。这项研究创造了一个免费的划痕分析应用程序，并提供了 CS-PRP 植入物有望用于治疗伤口的证据。