Abstract

Objectives

Cardiac transplantation is an effective treatment for advanced heart disease and protection of the donor organ is directly associated with post-transplantation outcomes. Cardioplegic strategies intend to protect the donor heart against ischemic injury during transplantation procedures. In our study, the effects of three different cardioplegia solutions were evaluated in a rat heart donor model in terms of cellular base. Design. Cardioplegia solutions as St. Thomas, del Nido or Custodiol were administered to male Wistar albino rats until cardiac arrest. Arrested hearts were excised and incubated in cold cardioplegia solutions for 4 h. Organ bath experiments were performed using the right ventricular free wall strips of the heart tissues. ATP, sialic acid, TNF-α levels and MMP-9 activities were measured in heart tissues. Incubation media were also used to measure TNF-α and troponin-I levels following organ baths experiments. Results. Custodiol administration led to reduced myocardial contraction (p < .05), decreased ATP levels (p < .001) and increased both TNF-α levels (p < .05), and MMP-9 activity (p < .05). Additionally, troponin-I and TNF-α levels in media were significantly increased (p < .05), TNF-α levels were positively correlated with MMP-9 activities (r = .93, p = .007) and negatively correlated with ATP levels (r = −.91, p = .01) in the Custodiol group. Also, MMP-9 activities were negatively correlated with ATP levels (r = −.90, p = .01) Conclusion. Custodiol cardioplegia cannot prevent functional and cellular damage in donor heart tissue. St. Thomas or del Nido cardioplegia could result in superior functional and biochemical improvement during transplantation procedures. In this respect, these cardioplegic solutions may be more advantageous as cellular and functional.

中文翻译：

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在供体心脏的大鼠模型中，St. Thomas 和 del Nido 心脏停搏液优于 Custodiol 心脏停搏液

摘要

目标

心脏移植是晚期心脏病的有效治疗方法，供体器官的保护与移植后结果直接相关。心脏停搏策略旨在保护供体心脏在移植过程中免受缺血性损伤。在我们的研究中，在大鼠心脏供体模型中根据细胞基础评估了三种不同心脏停搏液的效果。设计。将 St. Thomas、del Nido 或 Custodiol 等心脏麻痹溶液施用于雄性 Wistar 白化病大鼠，直至心脏骤停。切除被逮捕的心脏并在冷的心脏停搏液中孵育 4 小时。使用心脏组织的右心室游离壁条进行器官浴实验。测量心脏组织中的 ATP、唾液酸、TNF-α 水平和 MMP-9 活性。在器官浴实验后，孵育培养基也用于测量 TNF-α 和肌钙蛋白-I 水平。结果。Custodiol 给药导致心肌收缩减少 ( p  < .05)、ATP 水平降低 ( p  < .001) 并增加了 TNF-α 水平 ( p  < .05) 和 MMP-9 活性 ( p < .05)。此外，培养基中的肌钙蛋白-I 和 TNF-α 水平显着增加（p  < .05），TNF-α 水平与 MMP-9 活性呈正相关（r  = .93，p  = .007），与 ATP 呈负相关Custodiol 组中的水平 ( r = -.91, p  = .01)。此外，MMP-9 活性与 ATP 水平呈负相关 ( r = -.90, p  = .01)结论。Cutodiol 心脏停搏液不能防止供体心脏组织的功能和细胞损伤。St. Thomas 或 del Nido 心脏停搏液可在移植过程中产生卓越的功能和生化改善。在这方面，这些心脏停搏液在细胞和功能方面可能更有利。