Abstract

The aim of the current study was to develop the phytosomal gel of aloe vera extract for improved topical delivery. Aloe vera extract loaded phytosomal system was developed by fixing the amount of aloe vera extract and ethanol and by varying the concentration of lecithin (0.15–0.25% w/v) and speed of rotation (80–120 rpm). Different formulation batches were prepared as per the Design expert software. A 2² Factorial design was applied to optimize the formulation on the basis of vesicular size and entrapment efficiency. Developed formulations were evaluated for vesicular size, entrapment efficiency, PDI, zeta potential and in-vitro release. Further stability studies were also performed. For the optimized formulation (F09), vesicular size, entrapment efficiency and PDI were found as 123.1 ± 1.44 nm, 95.67 ± 0.27% and 0.98 ± 0.06. Zeta potential of −11.9 mV and drug release of 56.91 ± 4.1% obtained in 24 h. Drug release kinetics from the phytosomes follows Higuchi model. TEM micrograph confirms the uniform structure of phytosomes. Phytosomal gel of optimized phytosomal formulation (F09) was developed with 1% Carbopol 934 and physically characterized on the basis of pH, viscosity, homogeneity and drug content. Ex-vivo permeation study showed the better permeation and flux profile of phytosomal gel with the conventional aloe vera extract gel. Also, studies on phytosomal formulation and gel showed stability up-to 3 months. Thus overall, it can be concluded that the phytosomal gel is a good carrier for topical delivery of herbal extract such as aloe vera.

摘要

当前研究的目的是开发芦荟提取物的植物体凝胶以改善局部给药。通过固定芦荟提取物和乙醇的量以及改变卵磷脂的浓度（0.15-0.25% w/v）和旋转速度（80-120 rpm），开发了负载芦荟提取物的植物体系统。根据设计专家软件制备不同的配方批次。应用 2 ²因子设计以根据囊泡大小和包封率优化配方。对开发的制剂进行了囊泡大小、包封率、PDI、zeta 电位和体外评估释放。还进行了进一步的稳定性研究。对于优化的配方 (F09)，发现囊泡大小、截留效率和 PDI 为 123.1 ± 1.44 nm、95.67 ± 0.27% 和 0.98 ± 0.06。在 24 小时内获得 -11.9 mV 的 Zeta 电位和 56.91 ± 4.1% 的药物释放。来自植物体的药物释放动力学遵循 Higuchi 模型。TEM 显微照片证实了植物体的均匀结构。使用 1% Carbopol 934 开发了优化植物体配方 (F09) 的植物体凝胶，并根据 pH、粘度、均匀性和药物含量进行物理表征。离体渗透研究表明，与传统芦荟提取物凝胶相比，植物体凝胶具有更好的渗透性和通量分布。此外，对植物体配方和凝胶的研究表明稳定性长达 3 个月。因此，总的来说，可以得出结论，植物体凝胶是用于局部递送诸如芦荟的草本提取物的良好载体。