Panax notoginseng (Burkill) F.H.Chen (Araliaceae), of which the dry root and rhizome are precious traditional Chinese medicine, suffers severely from diseases during planting. Essential oils (EOs) with antimicrobial activity are a possibility for the development of green pesticides. We extracted EOs from Pogostemon cablin (Blanco) Benth. and Eupatorium fortunei Turcz., respectively and tested their inhibitory rates on fungi isolated from diseased P. notoginseng by the Oxford cup method. The compounds of the EO were identified by GC/MS and the minimum inhibitory concentrations (MICs) of the EOs and their main components were evaluated by the 96‐well plate method. We also mixed P. cablin EO, E. fortunei EO and hymexazol in pairs to explore whether their combinations produce stronger antifungal effects than individual components. Finally, we evaluated the effects of the EOs against Fusarium oxysporum in vivo. P. cablin EO and E. fortunei EO exhibited different antifungal activities against fungi, with the inhibitory rates of 21.02 %–100 % and 54.84 %–100 % and MICs of 0.07–0.88 mg/mL and 0.20–1.17 mg/mL, respectively. Pogostone (24.96 %) and thymol (15.64 %) were the major compounds of P. cablin EO and E. fortunei EO, respectively, and they exhibited stronger antifungal activities than EOs, with MICs of 0.008–0.078 mg/mL and 0.12–0.31 mg/mL, respectively. Moreover, hymexazol was mixed with E. fortunei EO, and the inhibitory effect against Cylindrocarpon destructans was enhanced with a synergistic effect. The disease incidence and disease index of EO treatments decreased significantly in vivo. Based on our study, P. cablin EO and E. fortunei EO have great potential to be developed into green fungicides for use in agriculture to control diseases of P. notoginseng.

中文翻译：

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黄芪和紫茎泽兰两种精油及其主要成分对三七分离真菌的生物活性

三七的干根和根茎是名贵中药材，在种植过程中病害严重。具有抗菌活性的精油 (EO) 是开发绿色农药的可能性。我们从 Pogostemon cablin (Blanco) Benth 中提取了 EO。和 Eupatorium Fortunei Turcz.，并通过牛津杯法测试了它们对从患病三七分离的真菌的抑制率。EO的化合物通过GC/MS进行鉴定，EO及其主要成分的最低抑菌浓度（MIC）通过96孔板法进行评估。我们还将 P. cablin EO、E. Fortunei EO 和 hymexazol 成对混合，以探索它们的组合是否比单个成分产生更强的抗真菌作用。最后，我们在体内评估了 EOs 对尖孢镰刀菌的影响。P. cablin EO 和 E. Fortunei EO 对真菌表现出不同的抗真菌活性，抑制率分别为 21.02 %–100 % 和 54.84 %–100 %，MIC 分别为 0.07–0.88 mg/mL 和 0.20–1.17 mg/mL . Pogostone (24.96 %) 和百里酚 (15.64 %) 分别是 P. cablin EO 和 E. Fortunei EO 的主要化合物，它们表现出比 EO 更强的抗真菌活性，MIC 为 0.008-0.078 mg/mL 和 0.12-0.31毫克/毫升，分别。此外，将hymexazol与E. Fortunei EO混合，增强了对Cylindrocarpon destructans的抑制作用，并具有协同作用。EO治疗的发病率和疾病指数在体内显着下降。根据我们的研究，P. cablin EO 和 E.