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Centrodinium punctatum (Dinophyceae) produces significant levels of saxitoxin and related analogs
Harmful Algae ( IF 6.6 ) Pub Date : 2020-11-13 , DOI: 10.1016/j.hal.2020.101923
Hyeon Ho Shin , Zhun Li , Damien Réveillon , Georges-Augustin Rovillon , Kenneth Neil Mertens , Philipp Hess , Hyun Jung Kim , Jihoon Lee , Kyun-Woo Lee , Daekyung Kim , Bum Soo Park , Jinik Hwang , Min Ho Seo , Weol Ae Lim

Centrodinium punctatum is a fusiform dinoflagellate with a global marine distribution. Due to a close phylogenetic relationship of one C. punctatum strain to Alexandrium species, toxin production of this C. punctatum strain was assessed using liquid chromatography coupled to tandem mass spectrometry. The paralytic shellfish toxin (PST) profile of C. punctatum was dominated by six analogs, i.e. STX (30%), GTX-1 (20%) and neoSTX (24%), followed by GTX-2 (9%), GTX-4 (9%) and GTX-3 (8%); deoxy-STX was also putatively identified while no gymnodimines, spirolides or goniodomins were detected. This is the first record of C. punctatum producing saxitoxins. The estimated cellular toxicity was rather elevated, between 91 and 212 pg cell−1 (or 259 and 601 fmol cell−1). When considering the toxicity equivalent factors, results suggest that this species can produce high cellular toxicity compared to other STX-producing dinoflagellates. Morphological details of the sulcal area and the hypotheca of Centrodinium punctatum were re-examined by scanning electron microscopy (SEM); this revealed that in the sulcal area, the left posterior sulcal plate (Ssp) is larger and longer than the left posterior sulcal plate and extended into the hypotheca. Based on the morphological observation, a revised interpretation of the sulcus and hypotheca is proposed.



中文翻译:

角点odi(Dinophyceae)产生大量的毒素和相关类似物

角点odi是一种梭形鞭毛藻,在全球范围内分布。由于一种点状梭菌菌株与亚历山大藻物种之间具有密切的系统发育关系,因此使用液相色谱-串联质谱联用仪评估了这种点状梭菌菌株的毒素产生。点状梭菌的麻痹性贝类毒素(PST)谱由六个类似物占主导,即STX(30%),GTX-1(20%)和neoSTX(24%),然后是GTX-2(9%),GTX -4(9%)和GTX-3(8%);还可以鉴定出脱氧STX,而未检测到裸子草碱,螺内酯或促性腺激素。这是C. punctatum的第一记录产生毒素。估计的细胞毒性相当高,介于91和212 pg细胞-1之间(或259和601 fmol细胞-1之间)。当考虑毒性当量因素时,结果表明,与其他产生STX的鞭毛鞭毛藻相比,该物种可产生高细胞毒性。通过扫描电子显微镜(SEM)重新检查状中心点的沟壁形态和假说。这表明在沟区域,左后沟板(Ssp)比左后沟板大和长,并延伸到假性囊膜。基于形态学观察,提出了对沟和假说的修订解释。

更新日期:2020-12-07
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