Passiflora cincinnata stands out among Passifloraceae because of its medicinal properties and its resistance to pathogens, which promotes its use as a rootstock for other Passiflora species. A valuable strategy for obtaining disease-resistant passion fruit plants is represented by genetic transformation; however, this requires efficient regeneration. Here, we aimed to establish an efficient protocol for generating transgenic passion fruit plants using sonication-assisted Agrobacterium-mediated transformation of somatic embryos. The latter were obtained from anthers, sonicated, and exposed to an Agrobacterium suspension for 15 or 30 s. As a comparison, non-sonicated embryos were also exposed to the same bacterial treatment, whereas non-infected embryos were used as controls. Plant identity was confirmed by PCR, qPCR, and histochemical assay. Transgenic plants were obtained at higher rates in the treatments applying sonication. Overall, 171 plantlets were regenerated, 38 of which showed stable uidA reporter gene expression. Of these 38 transgenic plants, 22 (57.89%) and 13 (34.21%) were obtained by sonication-assisted Agrobacterium-mediated transformation for 30 s and 15 s, respectively, whereas the remaining 3 (7.89%) were exposed for 30 s but without prior sonication. Our results indicate that sonication-assisted Agrobacterium-mediated transformation for 30 s enhanced transformation efficiency in P. cincinnata. We believe that this system will allow for more efficient production of transgenic passion fruit plants.

西番莲因其药用特性和对病原体的抗性而在西番莲科中脱颖而出，从而促进了其作为西番莲其他物种的砧木的用途。基因转化代表了获得抗病百香果植物的一种有价值的策略。但是，这需要有效的再生。在这里，我们旨在建立一种有效的协议，以使用声波辅助农杆菌介导的体细胞胚转化来产生转基因西番莲果植物。后者是从花药中获得的，经超声处理并暴露于农杆菌中悬置15或30 s。作为比较，未超声处理的胚也接受相同的细菌处理，而未感染的胚用作对照。通过PCR，qPCR和组织化学测定法确认植物身份。在应用超声处理的处理中，以更高的速率获得了转基因植物。总体上，再生了171株小植株，其中38株显示了稳定的uidA报告基因表达。在这38种转基因植物中，分别通过声波辅助农杆菌介导的转化30 s和15 s获得了22（57.89％）和13（34.21％），而其余​​3（7.89％）暴露了30 s，但是无需事先超声处理。我们的结果表明，超声辅助农杆菌介导的转化30 s提高了肉桂的转化效率。我们相信，该系统将使转基因百香果植物的生产更为有效。