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Expression difference of P450–1 and P450–4 between G- and F-groups of Fusarium fujikuroi
European Journal of Plant Pathology ( IF 1.8 ) Pub Date : 2020-11-13 , DOI: 10.1007/s10658-020-02133-3
W. X. Bao , S. Inagaki , S. Tatebayashi , S. Sultana , M. Shimizu , K. Kageyama , H. Suga

Fusarium fujikuroi is the pathogen of rice bakanae disease and has been intensively studied for gibberellin (GA) production. F. fujikuroi is phylogenetically subclassified into G- and F-groups, which differ in GA and fumonisin production. A higher amount of GA is produced by the G-group than the F-group. A previous study found that the GA production difference between the G-group strain Gfc0801001 and the F-group strain Gfc0825009 was due to the GA gene cluster. In this study, higher expression of P450–1 and P450–4 in the GA gene cluster were detected in G-group strains than in F-group strains. The nucleotide sequence of the bidirectional promoter region between P450–1 and P450–4 and expression of P450–1 and P450–4 were compared between G- and F-group strains. The promoter sequence was identical among G-group strains while nucleotide substitutions were detected at various locations in F-group strains. Most F-group strains did not share the same substitutions. These results combined with the ancestral position of the F-group in the phylogenetic tree suggest that the population carrying highly expressed sequence generates the G-group rather than the low expression of P450–1 and P450–4 in the F-group due to a specific mutation occured in G-group.



中文翻译:

富士镰刀菌G和F组P450-1和P450-4的表达差异

富士镰刀菌是水稻巴卡内氏病的病原体,并已针对赤霉素(GA)的生产进行了深入研究。藤本镰刀菌在系统发生上亚分类为G-和F-组,它们在GA和伏马菌素的生产上有所不同。G-基团产生的GA量高于F-基团。先前的研究发现,G组菌株Gfc0801001和F组菌株Gfc0825009之间的GA产量差异是由于GA基因簇引起的。在这项研究中,在G基因组中检测到的GA基因簇中P450-1P450-4的表达高于F组。P450-1P450-4之间双向启动子区域的核苷酸序列比较了G和F组菌株的P450-1P450-4的表达。在G-组菌株中启动子序列是相同的,而在F-组菌株的各个位置检测到核苷酸取代。大多数F组菌株没有相同的取代。这些结果与F-group在系统树中的祖先位置相结合,表明携带高表达序列的种群产生了G-group,而不是由于F-group中P450-1P450-4的低表达。G组发生特异性突变。

更新日期:2020-11-13
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