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Emergence of carbapenemase-producing and colistin resistant Klebsiella pneumoniae ST101 high-risk clone in Turkey.
Acta Microbiologica et Immunologica Hungarica ( IF 1.5 ) Pub Date : 2020-11-09 , DOI: 10.1556/030.2020.01275
Gülşen Hazırolan 1 , Alper Karagöz 2
Affiliation  

Carbapenemase-producing and colistin resistant Klebsiella pneumoniae has become a worldwide healthcare problem. This study describes molecular characterization of carbapenemase-producing and colistin resistant clinical K. pneumoniae isolates.A total of 93 non-replicate carbapenem and colistin resistant K. pneumoniae were recovered from clinical specimens in a university hospital during 2017-2019. Detection of blaOXA-48, blaKPC, blaNDM-1, blaIMP, blaVIM-1 and mcr-1, -2, -3, -4, -5, -6, -7, and -8 genes was performed by PCR. The bacterial isolates were assigned to clonal lineages by pulsed-field gel electrophoresis (PFGE) and multilocus sequence typing (MLST).All isolates harbored blaOXA-48 and only two isolates harbored blaOXA-48, and blaNDM-1 genes together. In colistin resistant K. pneumoniae, mcr-1 was detected in two (2.1%) isolates. Ninety three isolates of K. pneumoniae were categorized into three clusters and five pulsotypes. MLST revealed two different sequence types, ST101 (89/93) and ST147 (4/93).In our study ST101 was found to be a significantly dominant clone carrying blaOXA-48 and among our strains a low frequency of mcr-1 gene was determined. The emergence of colistin resistance was observed in K. pneumoniae ST101 isolates. ST101 may become a global threat in the dissemination of carbapenem and colistin resistance.

中文翻译:

土耳其出现产碳青霉烯酶和耐粘菌素的肺炎克雷伯菌 ST101 高危克隆。

产碳青霉烯酶和耐粘菌素的肺炎克雷伯菌已成为世界范围的医疗保健问题。本研究描述了产碳青霉烯酶和粘菌素耐药的临床肺炎克雷伯菌分离株的分子特征。2017-2019 年期间,从某大学医院的临床标本中回收了 93 株非重复的碳青霉烯和粘菌素耐药肺炎克雷伯菌。blaOXA-48、blaKPC、blaNDM-1、blaIMP、blaVIM-1 和 mcr-1、-2、-3、-4、-5、-6、-7 和 -8 基因的检测通过 PCR 进行。通过脉冲场凝胶电泳 (PFGE) 和多位点序列分型 (MLST) 将细菌分离株分配到克隆谱系。所有分离株都含有 blaOXA-48,只有两个分离株含有 blaOXA-48 和 blaNDM-1 基因。在耐粘菌素的肺炎克雷伯菌中,在两个 (2.1%) 分离株中检测到 mcr-1。九十三株肺炎克雷伯菌被分为三个簇和五个脉冲型。MLST 揭示了两种不同的序列类型,ST101 (89/93) 和 ST147 (4/93)。在我们的研究中,ST101 被发现是携带 blaOXA-48 的显性显性克隆,在我们的菌株中,低频率的 mcr-1 基因是决定。在肺炎克雷伯菌 ST101 分离株中观察到粘菌素耐药性的出现。ST101 可能成为碳青霉烯类和粘菌素耐药性传播的全球威胁。在肺炎克雷伯菌 ST101 分离株中观察到粘菌素耐药性的出现。ST101 可能成为碳青霉烯类和粘菌素耐药性传播的全球威胁。在肺炎克雷伯菌 ST101 分离株中观察到粘菌素耐药性的出现。ST101 可能成为碳青霉烯类和粘菌素耐药性传播的全球威胁。
更新日期:2020-11-14
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