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Controlling biocatalytic cascades with enzyme–DNA dynamic networks
Nature Catalysis ( IF 37.8 ) Pub Date : 2020-11-12 , DOI: 10.1038/s41929-020-00524-7
Chen Wang , Liang Yue , Itamar Willner

Cellular transformations, such as gene expression or temporal protein activities, are controlled by complex stimuli-responsive network circuitries regulated by enzymes, metabolites or transcription factors. Inspired by nature, extensive research efforts are directed to mimic these processes by in vitro chemical systems. Here we report on the assembly of constitutional dynamic networks (CDNs), composed of nucleic acid–enzyme conjugates, that act as modules for triggered, network-driven, biocatalytic cascades and for the intercommunication of network-guided biocatalytic cascades. Two CDNs were assembled—one network includes a constituent module functionalized with glucose oxidase and horseradish peroxidase in spatially close positions, and the second CDN includes a constituent module modified at sterically intimate positions with nicotinamide adenine dinucleotide and alcohol dehydrogenase. Biocatalytic cascades proceed in the two networks and, on the triggered reconfiguration of the CDNs, controlled and switchable biocatalytic cascades in the CDNs are demonstrated. The two CDNs are coupled, and the triggered feedback-driven intercommunication of the networks is realized.



中文翻译:

用酶-DNA动态网络控制生物催化级联反应

细胞转化,例如基因表达或时间蛋白活性,受酶,代谢物或转录因子调控的复杂刺激响应网络回路的控制。受自然界的启发,大量的研究工作试图通过体外化学系统模拟这些过程。在这里,我们报道由核酸-酶缀合物组成的组成动态网络(CDN)的组装,该网络充当触发的,网络驱动的生物催化级联以及网络引导的生物催化级联的相互通信的模块。组装了两个CDN,一个网络包含一个在空间上较近的位置用葡萄糖氧化酶和辣根过氧化物酶功能化的组成模块,第二CDN包括在结构上被烟酰胺腺嘌呤二核苷酸和醇脱氢酶修饰的组成模块。生物催化级联反应在两个网络中进行,并且在CDN触发重新配置后,证明了CDN中受控且可切换的生物催化级联反应。两个CDN耦合,实现了触发的反馈驱动的网络互通。

更新日期:2020-11-12
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