Abstract

Background

Diet macronutrient heterogeneity hinders animal studies’ data extrapolation from metabolic disorders to human diseases.

Objective

The present study aimed to evaluate different fat-diet compositions’ effect on inducing lipid/glucose metabolism alterations in mice.

Methods

Swiss male mice were fed for 12 weeks with five different diets: Standard Diet (ST), American Institute of Nutrition 93 for growth (AIN93G) high-butter/high-sugar (HBHS), high-lard/high-sugar (HLHS), and high-oil/high-sugar diet (soybean oil) (HOHS). Several parameters, such as serum biochemistry, histology, and liver mRNA expression, were accessed.

Results

The main findings revealed that the HLHS diet dramatically altered liver metabolism inducing hepatic steatosis and increased total cholesterol, triglycerides, VLDL, increasing liver CCAAT/enhancer binding protein (CEBP-α), Acetyl-CoA carboxylase (ACC) and Catalase (CAT) mRNA expression. Moreover, the HLHS diet increased glucose intolerance and reduced insulin sensitivity.

Conclusions

High-fat/high-sugar diets are efficient to induce obesity and metabolic syndrome-associated alterations, and diets enriched with lard and sugar showed more effective results.

中文翻译：

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膳食脂肪的比较研究：猪油和糖作为更好的肥胖和代谢综合征小鼠模型

摘要

背景

饮食宏量营养素的异质性阻碍了动物研究从代谢紊乱到人类疾病的数据外推。

客观的

本研究旨在评估不同脂肪饮食组合物对诱导小鼠脂质/葡萄糖代谢改变的影响。

方法

用五种不同的饮食喂养瑞士雄性小鼠 12 周：标准饮食 (ST)、美国营养研究所 93 生长 (AIN93G) 高黄油/高糖 (HBHS)、高猪油/高糖 (HLHS)和高油/高糖饮食（豆油）（HOHS）。访问了几个参数，例如血清生化、组织学和肝脏 mRNA 表达。

结果

主要发现表明，HLHS 饮食会显着改变肝脏代谢，导致肝脂肪变性并增加总胆固醇、甘油三酯、VLDL，增加肝脏 CCAAT/增强子结合蛋白 (CEBP-α)、乙酰辅酶 A 羧化酶 (ACC) 和过氧化氢酶 (CAT) mRNA表达。此外，HLHS 饮食增加了葡萄糖耐受不良并降低了胰岛素敏感性。

结论

高脂肪/高糖饮食可有效诱发肥胖和代谢综合征相关的改变，富含猪油和糖的饮食显示出更有效的结果。