Abstract

Florida or Cuban royal palm (Roystonea regia (Kunth) O.F. Cook) is an important ornamental plant with many uses for humans. Royal palm trees grown in Muscat, Oman, were found exhibiting leaf yellowing, leaf decay starting on the mature leaves, and leaf stunting. Trunk tissue samples were collected and analysed with nested and semi-nested PCR assays for the 16S rRNA, translation elongation factor Tu (tuf), and immunodominant membrane protein (imp) genes, in order to characterize the phytoplasma associated with the affected trees. Sequence and phylogenetic analysis of the 16S rRNA, tuf, and imp genes indicated that the phytoplasma strains detected in the trees were identical and belonged to the 16SrII-D subgroup. This is the first report of an association between ‘Candidatus Phytoplasma asutralasia’-related strain and R. regia.

摘要

佛罗里达或古巴皇家棕榈（Roystonea regia (Kunth) OF Coo​​k）是一种重要的观赏植物，对人类有多种用途。发现生长在阿曼马斯喀特的皇家棕榈树表现出叶子变黄、成熟叶子开始腐烂和叶子发育不良。收集树干组织样本，并使用巢式和半巢式 PCR 分析对 16S rRNA、翻译延伸因子 Tu ( tuf ) 和免疫显性膜蛋白 ( imp ) 基因进行分析，以表征与受影响树木相关的植原体。16S rRNA、tuf 和 imp 的序列和系统发育分析基因表明在树木中检测到的植原体菌株是相同的，属于 16SrII-D 亚群。这是关于' Candidatus Phytoplasma asutralasia '相关菌株与R. regia之间关联的第一份报告。