Abstract

The complete chloroplast genome of Populus deltoides was characterized by reference-based assembly using whole-genome sequencing data. The total chloroplast genome size of Populus deltoides included a pair of inverted repeat regions (IRs) of 27,649 bp each, a small single-copy region (SSC) of 16,563 bp, and large single-copy region (LSC) of 85,096 bp, which was 156,957 bp in length. A total of 109 genes were predicted from the chloroplast genome, including 83 protein-coding genes, 22 tRNA genes, and four rRNA genes. The GC content of chloroplast genome for Populus deltoides was 36.68%. The phylogenetic analysis based on the reported chloroplast genomes of Populus showed that the chloroplast of the Populus deltoides is most closely related to the Populus fremontii. The complete chloroplast genome of Populus deltoides provides new insights into Populus evolutionary and genomic studies.

摘要

利用全基因组测序数据，通过基于参考的组装对胡杨的完整叶绿体基因组进行了表征。美洲黑杨的总叶绿体基因组大小包括一对分别为27,649 bp的反向重复区域（IR），一个小的单拷贝区域（SSC）为16,563 bp和一个大的单拷贝区域（LSC）为85,096 bp，其中长度为156,957 bp。从叶绿体基因组预测总共有109个基因，包括83个蛋白质编码基因，22个tRNA基因和4个rRNA基因。毛白杨叶绿体基因组的GC含量为36.68％。基于报道的胡杨叶绿体基因组学的系统发育分析结果表明，毛白杨的叶绿体与fremontii的杨亲缘关系最密切。毛白杨的完整叶绿体基因组提供了对白杨进化和基因组研究的新见识。