Abstract

The mason bee, Osmia pedicornis Cockerell, 1919, which is importantly used as the pollinator, particularly for apples in Korea. We sequenced the mitochondrial genome (mitogenome) of O. pedicornis as an initial study for species identification and subsequent population genetic study. The size of the incomplete genome was 14,505 bp, excluding the trnA, trnC, and the A + T-rich region that were unable to sequence, but including partially sequenced trnM and srRNA. The genome included typical sets of protein-coding genes (PCGs), rRNA genes, and one non-coding region, tRNAs, excluding two unidentified tRNAs. Although positions of the two tRNAs that were not sequenced are unknown the gene arrangement of O. pedicornis mitogenome has the tRNA arrangement, trnM-trnQ-trnI, at the A + T-rich region and ND2 junction that differed from that of previously published O. excavate, which has trnA-trnQ-trnI arrangement at the junction. Phylogenetic analyses were performed using concatenated sequences of the 13 PCGs genes and the maximum likelihood method with the inclusion of a total of 12 mitogenome sequences belonging to three families in the superfamily Apoidea. Current O. pedicornis was placed as the sister to the O. bicornis, with the highest nodal support. The Apidae and Megachilidae were placed as the sister group, with the placement of Colletidae as the basal lineage for the group with the highest nodal support.

摘要

梅森蜜蜂Osmia pedicornis Cockerell，1919年，它很重要地用作传粉媒介，特别是在韩国的苹果中。我们对O. pedicornis的线粒体基因组（有丝分裂基因组）进行了测序，作为物种鉴定和后续种群遗传研究的初步研究。不完整基因组的大小为14,505 bp，不包括无法测序的trnA，trnC和富含A + T的区域，但包括部分测序的trnM和srRNA。基因组包括典型的蛋白质编码基因（PCG），rRNA集基因，一个非编码区，tRNA，不包括两个未鉴定的tRNA。尽管尚无两个未测序的tRNA的位置，但O. pedicornis有丝分裂基因组的基因排列在富含A + T的区域和ND2交界处的tRNA排列trnM - trnQ - trnI与先前发表的O挖掘，其中有trnA - trnQ - trnI交界处的安排。系统发育分析是使用13个PCGs基因的串联序列和最大似然法进行的，其中包括超家族Apoidea中属于三个家族的总共12个有丝分裂基因组序列。当前的O. pedicornis被放置为Bi。O. bicornis的姐妹，具有最高的节点支持度。Apidae和Megachilidae被放置为姐妹组，而Colletidae被放置为具有最高节点支持率的组的基础血统。