Green tea contains bioactive compounds, such as polyphenols, responsible for its health‐promoting effects, including antiobesity and antidiabetic effects. We previously reported that ultra‐sonication extraction (UE) could efficiently increase the extraction yield of green tea compounds. In the present study, we found that the extract obtained using UE contained higher phenolic and flavonoid contents than that obtained using the conventional method. We therefore considered the extract as a bioactive metabolite‐rich functional green tea extract (BMF‐GTE), and tested its glucose‐lowering effect by generating an adipocyte cell line stably expressing 7myc‐GLUT4‐GFP. We found that BMF‐GTE treatment increased GLUT4 translocation to the plasma membrane. Moreover, BMF‐GTE administration attenuated weight gain in mice fed a high‐fat diet (HFD). Importantly, HFD‐induced glucose tolerance was ameliorated in the mice receiving BMF‐GTE. Therefore, we conclude that BMF‐GTE worked against obesity and diabetes, at least partially, by enhancing GLUT4 translocation in adipocytes.

中文翻译：

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通过超声法提取的绿茶水浸膏，而不是常规方法，可促进脂肪细胞中的GLUT4膜移位，从而有效改善高脂饮食诱导的肥胖

绿茶含有生物活性化合物，例如多酚，具有促进健康的作用，包括抗肥胖和抗糖尿病作用。我们之前曾报道过超声提取（UE）可以有效地提高绿茶化合物的提取率。在本研究中，我们发现使用UE提取的提取物比使用常规方法提取的提取物中含有更高的酚类和类黄酮含量。因此，我们将提取物视为富含生物活性的功能性绿茶提取物（BMF-GTE），并通过生成稳定表达7myc-GLUT4-GFP的脂肪细胞系来测试其降糖效果。我们发现BMF‐GTE处理可增加GLUT4向质膜的转运。此外，BMF-GTE给药可减轻高脂饮食（HFD）喂养的小鼠的体重增加。重要的，在接受BMF-GTE的小鼠中，HFD诱导的葡萄糖耐量得到改善。因此，我们得出结论，BMF-GTE至少部分地通过增强脂肪细胞中的GLUT4易位而对抗肥胖和糖尿病。