With the wide application of neodymium oxide nanoparticles (NPs-Nd₂O₃) in various fields, their health hazards have aroused public concern in recent years. However, data regarding the cytotoxicity of NPs-Nd₂O₃ is limited. In this study, we investigated the function and mechanism of long-chain non-coding RNAs (lncRNAs) in NPs-Nd₂O₃-induced airway inflammation. Treatment with NPs-Nd₂O₃ induced an inflammatory response in human bronchial epithelial cells (16HBE) by upregulating the expression of interleukin-6 (IL-6) and interleukin-8 (IL-8). The levels of LDH and intracellular ROS in the cells treated by various doses of NPs-Nd₂O₃ also increased significantly. After treatment with 10 μg/ml NPs-Nd₂O₃, RNA microarray and real-time quantitative polymerase chain reaction (qRT-PCR) showed a significant upregulation of lncRNA loc105377478. Functional experiments suggested lncRNA loc105377478 enhanced the expression of IL-6, IL-8 and ROS in NPs-Nd₂O₃-treated 16HBE cells, and it was further demonstrated that lncRNA loc105377478 promoted the activation of NF-κB by negatively regulating ADIPOR1 expression. Moreover, the expression of IL-6 and IL-8 in NPs-Nd₂O₃-treated 16HBE cells was regulated by lncRNA loc105377478, which was mediated by the NF-κB signaling pathway. In conclusion, lncRNA loc105377478 promotes NF-κB activation by negatively regulating ADIPOR1 expression, thereby upregulating the expression of IL-6 and IL-8 in 16HBE cells treated with NPs-Nd₂O₃.

随着氧化钕纳米颗粒（NPs-Nd ₂ O ₃）在各个领域的广泛应用，近年来它们的健康危害引起了公众的关注。但是，关于NPs-Nd ₂ O ₃的细胞毒性的数据是有限的。在这项研究中，我们调查了NPs-Nd ₂ O ₃诱导的气道炎症中长链非编码RNA（lncRNA）的功能和机制。NPs-Nd ₂ O ₃处理通过上调白介素6（IL-6）和白介素8（IL-8）的表达在人支气管上皮细胞（16HBE）中引起炎症反应。不同剂量NPs-Nd处理的细胞中LDH和细胞内ROS的水平₂ O ₃也显着增加。用10μg/ ml NPs-Nd ₂ O _3处理后，RNA微阵列和实时定量聚合酶链反应（qRT-PCR）显示lncRNA loc105377478明显上调。功能实验表明lncRNA loc105377478增强了NPs-Nd ₂ O ₃处理的16HBE细胞中IL-6，IL-8和ROS的表达，并且进一步证明lncRNA loc105377478通过负调节ADIPOR1的表达促进了NF-κB的激活。 。此外，NPs-Nd ₂ O ₃中IL-6和IL-8的表达NF-κB信号通路介导的lncRNA loc105377478调控转染的16HBE细胞。总之，lncRNA loc105377478通过负调节ADIPOR1的表达来促进NF-κB的活化，从而上调NPs-Nd ₂ O ₃处理的16HBE细胞中IL-6和IL-8的表达。