As a new member of two-dimensional (2D) nanomaterials, black phosphorus (BP) has been considered as efficient photothermal therapy (PTT) agents owing to its excellent photothermal efficiency and biodegradability. Herein, a multifunctional nanoplatform based on black phosphorus nanosheets (BP NSs) was developed for chemo-photothermal synergistic cancer therapy. The BP NSs were successfully prepared by a liquid exfoliation technique. Doxorubicin (DOX), as a model drug, was loaded into the cavity of poly (amidoamine) (PAMAM) dendrimer using thin film hydration method. Then, PAMAM@DOX was coated on the surface of BP NSs using an electrostatic adsorption method that combined bath sonication with magnetic stirring. Hyaluronic acid (HA) was also modified onto the BP NS-PAMAM@DOX through electrostatic adsorption. PAMAM and HA layer could effectively isolate BP NSs from water and air to improve physiological stability. BP NSs and BP NS-PAMAM@DOX-HA were characterized by particle size, zeta potential, morphology, UV–vis-NIR absorption spectra, stability, photothermal performance and photothermal stability. This nanosystem exhibited a good pH and near infrared (NIR) dual-responsive drug release property. In addition, the obtained BP NS-PAMAM@D OX-HA nanocomposites possessed excellent PTT efficiency both in vitro and in vivo. The in vitro cell experiments suggested that the targeted BP NS-PAMAM@DOX-HA presented greater cytotoxicity and higher cellular uptake efficiency. Tumor xenograft model was established in BALB/C mice. The therapeutic effect of BP NS-PAMAM@DOX-HA was further augmented under 808 nm laser irradiation, displaying superior antitumor effect in comparison with chemotherapy or PTT alone. Such a biodegradable BP NS-based platform provide new insights for the rational design of PTT-based combinational cancer therapy.

作为二维（2D）纳米材料的新成员，黑磷（BP）由于其出色的光热效率和可生物降解性而被认为是有效的光热疗法（PTT）剂。在此，开发了基于黑磷纳米片（BP NSs）的多功能纳米平台，用于化学光热协同癌症治疗。通过液体剥离技术成功制备了BP NS。使用薄膜水合作用法将阿霉素（DOX）（作为模型药物）加载到聚（酰胺基胺）（PAMAM）树状聚合物的腔中。然后，使用静电吸附方法将PAMAM @ DOX涂覆在BP NSs的表面，该方法将浴超声处理与磁力搅拌相结合。透明质酸（HA）也通过静电吸附修饰到BP NS-PAMAM @ DOX上。PAMAM和HA层可以有效地将BP NSs与水和空气隔离，从而提高生理稳定性。BP NSs和BP NS-PAMAM @ DOX-HA的特征在于粒径，ζ电势，形态，UV-vis-NIR吸收光谱，稳定性，光热性能和光热稳定性。该纳米系统表现出良好的pH和近红外（NIR）双响应药物释放性能。此外，所获得的BP NS-PAMAM @ D OX-HA纳米复合材料在体外和体内均具有优异的PTT效率。体外细胞实验表明，靶向的BP NS-PAMAM @ DOX-HA表现出更大的细胞毒性和更高的细胞摄取效率。在BALB / C小鼠中建立肿瘤异种移植模型。在808 nm激光照射下，BP NS-PAMAM @ DOX-HA的治疗效果进一步增强，与单独使用化学疗法或PTT相比，具有更好的抗肿瘤作用。这种基于BP NS的可生物降解平台为基于PTT的联合癌症治疗的合理设计提供了新见识。