The combination therapy based on multifunctional nanocomposites has been considered as a promising approach to improve cancer therapeutic efficacy. Herein, we report targeted multi-functional poly(N-isopropylacrylamide) (PNIPAM)-based nanocomposites for synergistic chemo-photothermal therapy toward breast cancer cells. To increase the transition temperature, acrylic acid (AAc) was added in synthetic process of PNIPAM, showing that the intrinsic lower critical solution temperature was changed to 42 °C . To generate the photothermal effect under near-infrared (NIR) laser irradiation (808 nm), polypyrrole (ppy) nanoparticles were uniformly decorated in PNIPAM-AAc. Folic acid (FA), as a cancer targeting ligand, was successfully conjugated on the surplus carboxyl groups in PNIPAM network. The drug release of PNIPAM-ppy-FA nanocomposites was efficiently triggered in response to the temperature change by NIR laser irradiation. We also confirmed that PNIPAM-ppy-FA was internalized to MDA-MB-231 breast cancer cells by folate-receptor-mediated endocytosis and significantly enhanced cancer therapeutic efficacy with combination treatment of chemo-photothermal effects. Therefore, our work encourages further exploration of multi-functional nanocarrier agents for synergistic therapeutic approaches to different types of cancer cells.

基于多功能纳米复合材料的联合疗法已被认为是改善癌症治疗效果的一种有前途的方法。在此，我们提出有针对性的多功能聚（ñ-异丙基丙烯酰胺（PNIPAM）基纳米复合材料，用于对乳腺癌细胞进行协同化学光热疗法。为了提高转变温度，在PNIPAM的合成过程中添加了丙烯酸（AAc），这表明固有的较低临界溶液温度已更改为42°C。为了在近红外（NIR）激光辐照（808 nm）下产生光热效应，将聚吡咯（ppy）纳米颗粒均匀地装饰在PNIPAM-AAc中。叶酸（FA），作为一种癌症靶向配体，已成功地缀合到PNIPAM网络中的多余羧基上。PNIPAM-ppy-FA纳米复合材料的药物释放可有效响应NIR激光辐照引起的温度变化。我们还证实，PNIPAM-ppy-FA通过叶酸受体介导的内吞作用被内化到MDA-MB-231乳腺癌细胞中，并显着增强了化学光热效应的联合治疗的癌症治疗效果。因此，我们的工作鼓励进一步探索多功能纳米载体剂，以协同治疗不同类型癌细胞。