Sulforaphene (SFE), a major isothiocyanate in radish seeds, is a close chemical relative of sulforaphane (SFA) isolated from broccoli seeds and florets. The anti-proliferative mechanisms of SFA against cancer cells have been well investigated, but little is known about the potential anti-proliferative effects of SFE. In this study, we showed that SFE purified from radish seeds inhibited the growth of six cancer cell lines (A549, CHO, HeLa, Hepa1c1c7, HT-29, and LnCaP), with relative half maximal inhibitory concentration values ranging from 1.37 to 3.31 μg/mL. Among the six cancer cell lines, SFE showed the greatest growth inhibition against A549 lung cancer cells, where it induced apoptosis by changing the levels of poly(ADP-ribose) polymerase and caspase-3, -8, and -9. Our results indicate that SFE from radish seeds may have significant anti-proliferative potency against a broad range of human cancer cells via induction of apoptosis.

中文翻译：

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萝卜种子萝卜丁对A549细胞的增殖抑制作用

萝卜丁（SFE）是萝卜种子中的主要异硫氰酸盐，是从西兰花种子和小花中分离得到的萝卜硫素（SFA）的近亲。已经广泛研究了SFA对癌细胞的抗增殖机制，但对SFE的潜在抗增殖作用知之甚少。在这项研究中，我们表明从萝卜种子中纯化的SFE可以抑制6种癌细胞系（A549，CHO，HeLa，Hepa1c1c7，HT-29和LnCaP）的生长，相对最大抑制浓度值为1.37至3.31μg /毫升 在这六种癌细胞系中，SFE对A549肺癌细胞表现出最大的生长抑制作用，它通过改变多聚ADP-核糖聚合酶和caspase-3，-8和-9的水平诱导凋亡。