当前位置: X-MOL 学术Stem Cell Res. Ther. › 论文详情
Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)
Human menstrual blood-derived stem cells mitigate bleomycin-induced pulmonary fibrosis through anti-apoptosis and anti-inflammatory effects
Stem Cell Research & Therapy ( IF 7.5 ) Pub Date : 2020-11-11 , DOI: 10.1186/s13287-020-01926-x
Xin Chen 1 , Yi Wu 1 , Yanling Wang 1 , Lijun Chen 1 , Wendi Zheng 1 , Sining Zhou 1 , Huikang Xu 1 , Yifei Li 1 , Li Yuan 2 , Charlie Xiang 1
Affiliation  

Idiopathic pulmonary fibrosis is a kind of diffuse interstitial lung disease, the pathogenesis of which is unclear, and there is currently a lack of good treatment to improve the survival rate. Human menstrual blood-derived mesenchymal stem cells (MenSCs) have shown great potential in regenerative medicine. This study aimed to explore the therapeutic potential of MenSCs for bleomycin-induced pulmonary fibrosis. We investigated the transplantation of MenSCs in a pulmonary fibrosis mouse model induced by BLM. Mouse was divided into three groups: control group, BLM group, MenSC group. Twenty-one days after MenSC transplantation, we examined collagen content, pathological, fibrosis area in the lung tissue, and the level of inflammatory factors of serum. RNA sequence was used to examine the differential expressed gene between three groups. Transwell coculture experiments were further used to examine the function of MenSCs to MLE-12 cells and mouse lung fibroblasts (MLFs) in vitro. We observed that transplantation of MenSCs significantly improves pulmonary fibrosis mouse through evaluations of pathological lesions, collagen deposition, and inflammation. Transwell coculturing experiments showed that MenSCs suppress the proliferation and the differentiation of MLFs and inhibit the apoptosis of MLE-12 cells. Furthermore, antibody array results demonstrated that MenSCs inhibit the apoptosis of MLE-12 cells by suppressing the expression of inflammatory-related cytokines, including RANTES, Eotaxin, GM-CSF, MIP-1γ, MCP-5, CCL1, and GITR. Collectively, our results suggested MenSCs have a great potential in the treatment of pulmonary fibrosis, and cytokines revealed in antibody array are expected to become the target of future therapy of MenSCs in clinical treatment of pulmonary fibrosis.

中文翻译:

人经血干细胞通过抗凋亡和抗炎作用减轻博来霉素诱导的肺纤维化

特发性肺纤维化是一种弥漫性间质性肺疾病,其发病机制尚不清楚,目前缺乏良好的治疗方法来提高生存率。人类经血来源的间充质干细胞(MenSC)在再生医学方面显示出巨大的潜力。本研究旨在探讨 MenSCs 对博来霉素诱导的肺纤维化的治疗潜力。我们研究了 BLM 诱导的肺纤维化小鼠模型中 MenSC 的移植。将小鼠分为三组:对照组、BLM组、MenSC组。MenSC移植后21天,检测肺组织胶原含量、病理、纤维化面积以及血清炎症因子水平。RNA序列用于检查三组之间的差异表达基因。Transwell共培养实验进一步在体外检查MenSCs对MLE-12细胞和小鼠肺成纤维细胞(MLF)的功能。通过对病理病变、胶原沉积和炎症的评估,我们观察到MenSCs移植显着改善了小鼠的肺纤维化。Transwell共培养实验表明,MenSCs可抑制MLF的增殖和分化,并抑制MLE-12细胞的凋亡。此外,抗体阵列结果表明,MenSC 通过抑制炎症相关细胞因子(包括 RANTES、Eotaxin、GM-CSF、MIP-1γ、MCP-5、CCL1 和 GITR)的表达来抑制 MLE-12 细胞凋亡。总的来说,我们的结果表明MenSCs在治疗肺纤维化方面具有巨大的潜力,抗体阵列中揭示的细胞因子有望成为未来MenSCs在肺纤维化临床治疗中的治疗靶点。
更新日期:2020-11-12
down
wechat
bug