Frontiers in Physics ( IF 3.1 ) Pub Date : 2020-10-05 , DOI: 10.3389/fphy.2020.582956 Thompson Lu , Bahman Anvari
Ovarian cancer is the deadliest gynecological cancer in women. It is a highly metastatic disease with pelvis, regional lymph nodes, and peritoneal cavity as major sites for tumor deposits. Mechanical properties of ovarian cancer cells can play a major role in metastasis as the cells detach from the ovaries and undergo deformation during the metastatic process. Herein, we have characterized the viscoelastic properties of the plasma membrane of normal epithelial (IOSE364) and cancerous (SKOV3) ovarian cells by optical tweezers and quantitative phase imaging. Using optical tweezers, we obtained time-resolved force profiles associated with membrane tethers pulled from the cells. We used quantitative phase imaging to measure the diameter of membrane tethers, and subsequently, estimated the membrane bending modulus and membrane tension in the tether. Our results indicate that the force (190 ± 76 pN) (mean ± standard deviation) required to separate the membrane of SKOV3 cells from the cytoskeleton was significantly lower (
中文翻译:
光学镊子和定量相成像表征卵巢癌细胞膜的粘弹性。
卵巢癌是女性中最致命的妇科癌症。它是一种高度转移性疾病,骨盆,局部淋巴结和腹膜腔为肿瘤沉积的主要部位。卵巢癌细胞的机械特性在转移过程中起着重要作用,因为这些细胞会从卵巢脱离并在转移过程中发生变形。在这里,我们已经表征了正常镊子(IOSE364)和癌细胞(SKOV3)卵巢细胞质膜的粘弹性特性,通过光镊和定量相成像。使用光镊,我们获得了与时间间隔有关的力分布,这些力分布与从细胞中拉出的膜系绳有关。我们使用定量相位成像技术来测量膜系链的直径,然后估算系链中的膜弯曲模量和膜张力。