当前位置: X-MOL 学术Prog. Nat. Sci. Mater. Int. › 论文详情
Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)
Polymer coated nanodiamonds as gemcitabine prodrug with enzymatic sensitivity for pancreatic cancer treatment
Progress in Natural Science: Materials International ( IF 4.7 ) Pub Date : 2020-10-01 , DOI: 10.1016/j.pnsc.2020.10.011
Wanying Ye , Haijie Han , Huan Li , Qiao Jin , Yuzhou Wu , Sabyasachi Chakrabortty , Tanja Weil , Jian Ji

Abstract The fabrication of Gemcitabine (GEM) prodrug was reported to be an effective method to enhance its pancreatic cancer treatment efficiency. Here, a kind of nanocarbon-based materials, nanodiamond (ND), was selected as the nanocarrier of GEM, owing to its outstanding surface properties and non-cytotoxicity. The polyelectrolytes, polyethyleneimine and polyacrylic acid, were used to self-assemble outside ND surface through electrostatic forces, followed by attachment of polyethylene glycol to address better biocompatibility. GEM was conjugated with an enzyme-sensitive peptide gly-phe-leu-gly to build up the controlled release platform. From characterization results of dynamic laser scattering, zeta potential and transmission electron microscope, the significant improvement of ND stability in physiological condition was proved. Non-cytotoxicity of this functionalized ND carriers and cytotoxicity of the prodrug against BxPC-3 pancreatic cancer cells were indicated by methylthiazolyl tetrazolium (MTT) assay. In vivo experiments also revealed its superior anticancer effect compared with free GEM treatment. Therefore, the combination of polymer coated NDs with high surface capability and enzyme-responsive intracellular GEM release make it possible to realize higher treatment efficiency on pancreatic tumor therapy.

中文翻译:

聚合物包覆的纳米金刚石作为具有酶敏感性的吉西他滨前药用于胰腺癌治疗

摘要 据报道,吉西他滨(GEM)前药的制备是提高其胰腺癌治疗效率的有效方法。在这里,一种纳米碳基材料纳米金刚石(ND)因其优异的表面性能和非细胞毒性而被选为 GEM 的纳米载体。聚电解质聚乙烯亚胺和聚丙烯酸用于通过静电力在 ND 表面外自组装,然后附着聚乙二醇以解决更好的生物相容性。GEM 与酶敏感肽 gly-phe-leu-gly 结合以构建控释平台。从动态激光散射、zeta电位和透射电子显微镜的表征结果,证明了生理条件下ND稳定性的显着提高。这种功能化的 ND 载体的非细胞毒性和前药对 BxPC-3 胰腺癌细胞的细胞毒性通过甲基噻唑基四唑 (MTT) 测定表明。体内实验还揭示了与游离 GEM 治疗相比其优越的抗癌作用。因此,具有高表面能力的聚合物包覆的NDs和酶响应性细胞内GEM释放的结合使得在胰腺肿瘤治疗中实现更高的治疗效率成为可能。
更新日期:2020-10-01
down
wechat
bug