Photo-or optoacoustic imaging (OAI) allows quantitative imaging of target tissues. Using multi-wavelength illumination with subsequent ultrasound detection, it may visualize a variety of different chromophores at centimeter depth. Despite its non-invasive, label-free advantages, the precision of repeated measurements for clinical applications is still elusive. We present a multilayer analysis of n = 1920 imaging datasets obtained from a prospective clinical trial (NCT03979157) in n = 10 healthy adult volunteers. All datasets were analyzed for 13 single wavelengths (SWL) between 660 nm–1210 nm and five MSOT-parameters (deoxygenated/oxygenated/total hemoglobin, collagen and lipid) by a semi-automated batch mode software. Intraclass correlation coefficients (ICC) were good to excellent for intrarater (SWL: 0.82–0.92; MSOT-parameter: 0.72−0.92) and interrater reproducibility (SWL: 0.79−0.87; MSOT-parameter: 0.78−0.86), with the exception for MSOT-parameter lipid (interrater ICC: 0.56). Results were stable over time, but exercise-related effects as well as inter-and intramuscular variability were observed. The findings of this study provide a framework for further clinical OAI implementation.

光声或光声成像（OAI）可以对靶组织进行定量成像。将多波长照明与随后的超声波检测一起使用，可以在厘米深度可视化各种不同的生色团。尽管它具有无创，无标签的优点，但在临床应用中重复测量的精度仍然难以捉摸。我们提出了对n = 10位健康成人志愿者的前瞻性临床试验（NCT03979157）获得的n = 1920个成像数据集的多层分析。通过半自动批处理模式软件分析了所有数据集的660 nm–1210 nm之间的13个单波长（SWL）和5个MSOT参数（脱氧/氧化/总血红蛋白，胶原蛋白和脂质）。类内相关系数（ICC）对评估者而言好坏（SWL：0.82-0.92； MSOT参数：0.72--0。92）和间质可重复性（SWL：0.79-0.87; MSOT参数：0.78-0.86），MSOT参数脂质（interrater ICC：0.56）除外。结果随时间推移保持稳定，但观察到与运动有关的影响以及肌间和肌内变异性。这项研究的结果为进一步的临床OAI实施提供了框架。