We used antioxidant-containing nanoparticles (NPs) to treat acute hearing loss. Alpha-lipoic acid (ALA) served as the antioxidant; we employed Pluronic F127 to fabricate NPs. In vitro, ALA-NPs protected cells of the organ of Corti in HEI-OC1 mice, triggering nuclear translocation of NRF2 and increases in the levels of antioxidant proteins, including Nrf2, HO-1, SOD-1, and SOD-2. In vivo, the hearing of mice that received ALA-NP injections into the middle ear cavity was better preserved after induction of ototoxicity than in control animals. The cochlear Nrf2 level increased in test mice, indicating that the ALA-NPs protected hearing via the antioxidant mechanism observed in vitro. ALA-NPs effectively protected against acute hearing loss by activating the Nrf2/HO-1 pathway.

我们使用含抗氧化剂的纳米粒子 (NPs) 来治疗急性听力损失。α-硫辛酸 (ALA) 作为抗氧化剂；我们采用 Pluronic F127 来制造 NP。在体外，ALA-NPs 保护 HEI-OC1 小鼠 Corti 器官的细胞，触发 NRF2 的核易位并增加抗氧化蛋白的水平，包括 Nrf2、HO-1、SOD-1 和 SOD-2。在体内，在中耳腔中接受 ALA-NP 注射的小鼠的听力在诱导耳毒性后比对照动物更好。测试小鼠的耳蜗 Nrf2 水平增加，表明 ALA-NPs 通过体外观察到的抗氧化机制保护听力. ALA-NPs 通过激活 Nrf2/HO-1 通路有效地防止急性听力损失。