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The value of HPV E6/E7 mRNA quantitative analysis in distinguishing high-grade cervical squamous intraepithelial lesions from low-grade cervical squamous intraepithelial lesions
Journal of Virological Methods ( IF 3.1 ) Pub Date : 2020-11-11 , DOI: 10.1016/j.jviromet.2020.114014
Jixuan Liu 1 , Teng Yang 2 , Yubo Hu 3 , Cong Ye 4
Affiliation  

A quantitative assay for HPV E6/E7 mRNA may be a valuable tool for cervical cancer screening. The purpose of this study is to compare the expression levels of HPV E6/E7 mRNA in high-grade cervical squamous intraepithelial lesion (HSIL) and low-grade squamous intraepithelial lesions (LSIL) and to determine a new method that can be used to distinguish cervical squamous intraepithelial lesions. Routine cytology, HR-HPV E6/E7 mRNA, histology, and p16 immunohistochemistry were performed in tissues from 142 patients with cervical squamous intraepithelial lesions. Significant differences were observed between the E6/E7 mRNA copy number values between the LSIL and HSIL cases (Mann–Whitney U‐test, P < 0.001). The optimal cut-off value (≥9,222.00 copies/mL) was determined using the receiver operating characteristic curve to predict diagnostic performance. Out of the 161 samples tested in this study, four cases were classified cytologically as HSIL but had normal histology. The E6/E7 copy numbers in these cases were all higher than 9,222 copies/mL. Therefore, a quantitative assay for HPV E6/E7 mRNA may be a valuable tool that can be used to distinguish HSIL and LSIL, especially for those with HSIL, for which samples are not obtained by biopsy, or when HSIL is difficult to distinguish by morphology and p16 immunohistochemistry.



中文翻译:

HPV E6/E7 mRNA定量分析鉴别高级别宫颈鳞状上皮内病变与低级别宫颈鳞状上皮内病变的价值

HPV E6/E7 mRNA 的定量检测可能是宫颈癌筛查的重要工具。本研究的目的是比较高级别宫颈鳞状上皮内病变(HSIL)和低级别鳞状上皮内病变(LSIL)中HPV E6/E7 mRNA的表达水平,确定一种可用于区分宫颈鳞状上皮内病变(HSIL)的新方法。宫颈鳞状上皮内病变。对 142 名宫颈鳞状上皮内病变患者的组织进行常规细胞学、HR-HPV E6/E7 mRNA、组织学和 p16 免疫组化。在 LSIL 和 HSIL 病例之间的 E6/E7 mRNA 拷贝数值之间观察到显着差异(Mann-Whitney U 检验,P < 0.001)。最佳临界值(≥9,222.00 拷贝/mL)通过使用受试者工作特征曲线来预测诊断性能来确定。在本研究中测试的 161 个样本中,有 4 个病例在细胞学上被归类为 HSIL,但组织学正常。这些情况下的 E6/E7 拷贝数均高于 9,222 拷贝/mL。因此,HPV E6/E7 mRNA的定量检测可能是一种有价值的工具,可用于区分HSIL和LSIL,特别是对于HSIL患者,其样本无法通过活检获得,或者HSIL难以通过形态区分时和 p16 免疫组化。

更新日期:2020-12-30
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