Abstract The transfer of substances via the blood-placenta barrier is tightly regulated and critical for the fetus and the expecting mother. In case of necessary medical interventions during pregnancy a comprehensive knowledge about the interaction of the drugs with this barrier is indispensable. Therefore well-engineered test systems are needed and valuable transport systems are helpful. We developed an in vitro microfluidic blood-placenta barrier system consisting of the human trophoblast cell line BeWo and human primary placental pericytes. The integrity and stability of the model was verified by a permeability assay and immunocytochemistry. As potential drug carriers magnetic nanoparticles with various coatings were applied and their ability to pass the barrier was quantified by magnetic particle spectroscopy. We could demonstrate that up to 4% of negatively charged nanoparticles pass the barrier in a time-dependent manner.

中文翻译：

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带负电荷的磁性纳米粒子在连续流动条件下以时间依赖性方式通过血胎盘屏障

摘要 通过血胎盘屏障的物质转移受到严格调控，对胎儿和准妈妈至关重要。在怀孕期间进行必要的医疗干预的情况下，关于药物与该屏障相互作用的全面知识是必不可少的。因此，需要精心设计的测试系统，有价值的传输系统会有所帮助。我们开发了一种体外微流体血胎盘屏障系统，由人类滋养层细胞系 BeWo 和人类原代胎盘周细胞组成。通过渗透性测定和免疫细胞化学验证了模型的完整性和稳定性。应用具有各种涂层的磁性纳米粒子作为潜在的药物载体，并通过磁性粒子光谱法量化它们通过屏障的能力。