Abstract Conducting polymers are promising materials used to prepare electrochemical and optical detection platforms for bioanalytical systems. After conjugation of biomolecules onto electropolymerized monomers for preparation of multifunctional surfaces, they can be used to easily monitor small molecules, macromolecules, and cells. In this study, multifunctional monomer with indole (necessary for fluorescence detection of H2O2) and carboxyl functional groups (necessary for covalent immobilization of biological material) was synthesized, electropolymerized, modified with biomolecules (catalase) and then applied for the selective detection of target analyte (H2O2). Tryptophan sequestered dithione [3,2-b:2’,3’-d] pyrrole (DTP-Trp) was synthesized and electropolymerized on indium tin oxide (ITO) coated polyethylene terephthalate (PET) substrates, which is a transparent support. Afterward, catalase (CAT) was immobilized on the Poly(DTP-Trp) using a zero-length crosslinker, 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide hydrochloride (EDC), via covalent bonds between carboxyl groups of CAT and amino groups of Poly(DTP-Trp). Surface characterization of Poly(DTP-Trp)/CAT was done by scanning electron microscopy (SEM) and energy dispersive x-ray analysis (EDX). The presence of Poly(DTP-Trp)/CAT on ITO-PET surfaces were also confirmed by electrochemical methods to show the success of support modification. Determination of H2O2 was investigated using fluorescence spectrometry based on quenching mechanism of H2O2 on the fluorescence of tryptophan. After optimization of working conditions of Poly(DTP-Trp)/CAT, H2O2 detection in synthetic samples was done without any interference of the matrices.

中文翻译：

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过氧化氢酶共轭表面：基于色氨酸荧光猝灭对导电聚合物的 H2O2 检测

摘要 导电聚合物是用于制备生物分析系统电化学和光学检测平台的有前途的材料。在将生物分子偶联到电聚合单体上以制备多功能表面后，它们可用于轻松监测小分子、大分子和细胞。本研究合成了具有吲哚（荧光检测 H2O2 所必需）和羧基官能团（生物材料共价固定所必需）的多功能单体，经电聚合，用生物分子（过氧化氢酶）修饰，然后应用于目标分析物的选择性检测(H2O2)。色氨酸螯合二硫酮 [3,2-b:2',3'-d] 吡咯 (DTP-Trp) 在氧化铟锡 (ITO) 涂层聚对苯二甲酸乙二醇酯 (PET) 基材上合成和电聚合，这是一个透明的支持。然后，通过 CAT 的羧基和氨基之间的共价键，使用零长度交联剂 1-乙基-3-（3-二甲基氨基丙基）碳二亚胺盐酸盐（EDC）将过氧化氢酶（CAT）固定在聚（DTP-Trp）上。聚（DTP-Trp）组。Poly(DTP-Trp)/CAT 的表面表征通过扫描电子显微镜 (SEM) 和能量色散 X 射线分析 (EDX) 完成。电化学方法也证实了聚 (DTP-Trp)/CAT 在 ITO-PET 表面上的存在，以显示载体改性的成功。基于 H2O2 对色氨酸荧光的猝灭机制，使用荧光光谱法研究了 H2O2 的测定。在优化 Poly(DTP-Trp)/CAT 的工作条件后，合成样品中的 H2O2 检测在没有基质干扰的情况下进行。