Marine bivalves have been widely applied as environmental contamination bioindicators, although studies concerning tropical species are less available compared to temperate climate species. Assessments regarding Perna perna mytilid mussels, in particular, are scarce, even though this is an extremely important species in economic terms in tropical countries, such as Brazil. To this end, Perna perna mytilids were sampled from two tropical bays in Southeastern Brazil, one anthropogenically impacted and one previously considered a reference site for metal contamination. Gill metallothionein (MT), reduced glutathione (GSH), carboxylesterase (CarbE) and lipid peroxidation (LPO) were determined by UV−vis spectrophotometry, and metal and metalloid contents were determined by inductively coupled plasma mass spectrometry (ICP−MS). Metalloprotein metal detoxification routes in heat-stable cellular gill fractions were assessed by size exclusion high performance chromatography (SEC−HPLC) coupled to an ICP−MS. Several associations between metals and oxidative stress endpoints were observed at all four sampling sites through a Principal Component Analysis. As, Cd, Ni and Se contents, in particular, seem to directly affect CarbE activity. MT is implicated in playing a dual role in both metal detoxification and radical oxygen species scavenging. Differential SEC−HPLC−ICP−MS metal-binding profiles, and, thus, detoxification mechanisms, were observed, with probable As-, Cu- and Ni-GSH complexation and binding to low molecular weight proteins. Perna perna mussels were proven adequate tropical bioindicators, and further monitoring efforts are recommended, due to lack of data regarding biochemical metal effects in tropical species. Integrated assessments, as performed herein demonstrate, are invaluable in evaluating contaminated aquatic environments, resulting in more accurate ecological risk assessments.

尽管与温带气候物种相比，有关海洋物种的研究较少，但海洋双壳类动物已被广泛用作环境污染生物指示剂。关于评估翡翠翡翠mytilid蚌，尤其是稀缺的，即使这是在热带国家，如巴西经济上极其重要的物种。为此，佩尔纳佩尔纳从巴西东南部的两个热带海湾采集了甲壳类动物，其中一个受到人为影响，另一个以前被认为是金属污染的参考点。通过紫外可见分光光度法测定金属硫蛋白（MT），还原型谷胱甘肽（GSH），羧酸酯酶（CarbE）和脂质过氧化（LPO），并通过电感耦合等离子体质谱法（ICP-MS）测定金属和准金属的含量。通过与ICP-MS结合的尺寸排阻高效色谱（SEC-HPLC）评估了热稳定细胞g部分中的金属蛋白金属排毒途径。通过主成分分析在所有四个采样点观察到金属和氧化应激终点之间的几种关联。特别是，由于Cd，Ni和Se的含量似乎直接影响CarbE的活性。MT参与了金属排毒和清除自由基氧的双重作用。观察到了不同的SEC-HPLC-ICP-MS金属结合曲线，以及由此观察到的解毒机理，其中可能存在As-，Cu-和Ni-GSH络合并与低分子量蛋白质结合。多年生贻贝被证明是合适的热带生物指标，由于缺乏有关热带物种生化金属效应的数据，因此建议进一步监测。如本文所进行的综合评估表明，在评估受污染的水生环境方面具有不可估量的价值，从而可以进行更准确的生态风险评估。