Cnesterodon decemmaculatus is a Neotropical teleost fish frequently used in ecotoxicological evaluations, whose biology has been thoroughly studied. Although there is considerable information on its response to different toxicants, no range of reference values has been so far established for the different biological parameters proposed as biomarkers of effect or exposure. Moreover, no study has yet examined the possible influence of the metabolic status of the exposed animals on their response to toxic stress. Therefore, the aim of this work was to provide a first baseline for a set of bioenergetic biomarkers in C. decemmaculatus adults exposed to a control medium under previously standardized conditions, and to assess their possible intrinsic seasonal variability. The responses of the biomarkers obtained from the controls were contrasted with those from the reference toxicant (Cadmio-Cd) and receiving waters (surface waters of the Reconquista River RR, Buenos Aires Province, Argentina). We conducted four 12-day assays (one in each season) of exposure to control media, (reconstituted moderate hard water, MHW) and two assays of exposure to Cd in MHW and surface river water (RR) in both summer and autumn. The variables recorded were: Food intake (In), fecal production (F), specific assimilation (A) and cumulative mortality, oxygen extraction efficiency (OEE), specific metabolic rate (SMR), ammonia excretion (N), ammonia quotient (AQ) and scope for growth (SFG). The seasonal variation shown by some physiological parameters, points to the need for establishing a baseline obtained from standardized media, preferably on a seasonal basis. Moreover, SFG and A appeared as the most sensitive biomarkers, emphasizing the importance to consider the metabolic status of the test organisms for the appropriate interpretation of results from ecotoxicological studies performed under controlled experimental conditions. The obtained results provide useful information on C. decemmaculatus as model species in ecotoxicological bioassays involving biomarkers of early effect.

Cnesterodon decemmaculatus是常用于生态毒理学评估的新热带硬骨鱼类，其生物学已得到深入研究。尽管关于其对不同毒物的反应有相当多的信息，但到目前为止，对于提议作为效应或暴露生物标志物的不同生物学参数，尚未建立参考值范围。而且，还没有研究检查暴露动物的代谢状态对其毒性毒性反应的可能影响。因此，这项工作的目的是为蜕皮梭菌中的一组生物能生物标志物提供第一个基线。成人在先前标准化的条件下接触对照培养基，并评估其可能的内在季节性变化。从对照中获得的生物标志物的反应与参考毒物（Cadmio-Cd）和接受水（阿根廷布宜诺斯艾利斯省的Reconquista河RR的地表水）的反应进行了对比。我们进行了四次为期12天的试验（每个季节一次），分别暴露于对照介质（重配的中度硬水MHW）中，在夏季和秋季分别进行了两次MHW和地表河水（RR）中Cd暴露的测定。记录的变量为：食物摄入（In），粪便产生（F），比吸收（A）和累积死亡率，氧气提取效率（OEE），比代谢率（SMR），氨排泄（N），氨商（AQ） ）和增长范围（SFG）。一些生理参数显示的季节性变化表明需要建立从标准化媒体获得的基线，最好是在季节性基础上。此外，SFG和A似乎是最敏感的生物标志物，强调了在适当控制条件下进行生态毒理学研究结果的适当解释时，考虑测试生物的代谢状态的重要性。获得的结果提供了有关以下方面的有用信息 强调考虑受试生物的代谢状态以适当解释在受控实验条件下进行的生态毒理学研究结果的重要性。获得的结果提供了有关以下方面的有用信息 强调为了适当地解释在受控实验条件下进行的生态毒理学研究结果而考虑被测生物的代谢状态的重要性。获得的结果提供了有关以下方面的有用信息C. decemmaculatus是生态毒理生物测定中的模型物种，涉及早期效应的生物标记。