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Digital dipstick: miniaturized bacteria detection and digital quantification for the point-of-care
Lab on a Chip ( IF 6.1 ) Pub Date : 2020-11-10 , DOI: 10.1039/d0lc00793e
Emre Iseri 1 , Michael Biggel , Herman Goossens , Pieter Moons , Wouter van der Wijngaart
Affiliation  

Established digital bioassay formats, digital PCR and digital ELISA, show extreme limits of detection, absolute quantification and high multiplexing capabilities. However, they often require complex instrumentation, and extensive off-chip sample preparation. In this study, we present a dipstick-format digital biosensor (digital dipstick) that detects bacteria directly from the sample liquid with a minimal number of steps: dip, culture, and count. We demonstrate the quantitative detection of Escherichia coli (E. coli) in urine in the clinically relevant range of 102–105 CFU ml−1 for urinary tract infections. Our format shows 89% sensitivity to detect E. coli in clinical urine samples (n = 28) when it is compared to plate culturing (gold standard). The significance and uniqueness of this diagnostic test format is that it allows a non-trained operator to detect urinary tract infections in the clinically relevant range in the home setting.

中文翻译:

数字量油尺:用于现场护理的小型细菌检测和数字量化

已建立的数字生物测定格式,数字PCR和数字ELISA显示了检测极限,绝对定量和高复用能力。但是,它们通常需要复杂的仪器和大量的片外样品制备。在这项研究中,我们提出了一种量油尺形式的数字生物传感器(数字量油尺),该传感器可通过最少的步骤(浸入,培养和计数)直接从样品液体中检测细菌。我们证明了在尿道感染的临床相关范围10 2 –10 5 CFU ml -1中定量检测尿液中的大肠杆菌E. coli)。我们的格式显示出89%的灵敏度可检测大肠杆菌与平板培养(金标准)比较时,在临床尿液样本中(n = 28)。这种诊断测试格式的重要性和独特性在于,它允许未经培训的操作人员在家中临床相关范围内检测泌尿道感染。
更新日期:2020-11-12
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