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Indicator displacement assays (IDAs): the past, present and future
Chemical Society Reviews ( IF 46.2 ) Pub Date : 2020-11-10 , DOI: 10.1039/c9cs00538b
Adam C. Sedgwick 1, 2, 3, 4 , James T. Brewster 1, 2, 3, 4 , Tianhong Wu 5, 6, 7, 8, 9 , Xing Feng 5, 6, 7, 8, 9 , Steven D. Bull 1, 10, 11, 12 , Xuhong Qian 13, 14, 15, 16, 17 , Jonathan L. Sessler 1, 2, 3, 4 , Tony D. James 1, 10, 11, 12 , Eric V. Anslyn 1, 2, 3, 4 , Xiaolong Sun 5, 6, 7, 8, 9
Affiliation  

Indicator displacement assays (IDAs) offer a unique and innovative approach to molecular sensing. IDAs can facilitate the detection of a range of biologically/environmentally important species, provide a method for the detection of complex analytes or for the determination and discrimination of unknown sample mixtures. These attributes often cannot be achieved by traditional molecular sensors i.e. reaction-based sensors/chemosensors. The IDA pioneers Inouye, Shinkai, and Anslyn inspired researchers worldwide to develop various extensions of this idea. Since their early work, the field of indicator displacement assays has expanded to include: enantioselective indicator displacement assays (eIDAs), fluorescent indicator displacement assays (FIDAs), reaction-based indicator displacement assays (RIAs), DimerDye disassembly assays (DDAs), intramolecular indicator displacement assays (IIDAs), allosteric indicator displacement assay (AIDAs), mechanically controlled indicator displacement assays (MC-IDAs), and quencher displacement assays (QDAs). The simplicity of these IDAs, coupled with low cost, high sensitivity, and ability to carry out high-throughput automation analysis (i.e., sensing arrays) has led to their ubiquitous use in molecular sensing, alongside the other common approaches such as reaction-based sensors and chemosensors. In this review, we highlight the various design strategies that have been used to develop an IDA, including the design strategies for the newly reported extensions to these systems. To achieve this, we have divided this review into sections based on the target analyte, the importance of each analyte and then the reported IDA system is discussed. In addition, each section includes details on the benefit of the IDAs and perceived limitations for each system. We conclude this Tutorial Review by highlighting the current challenges associated with the development of new IDAs and suggest potential future avenues of research.

中文翻译:

指标位移测定(I​​DA):过去,现在和未来

指示剂位移测定(I​​DA)为分子传感提供了独特的创新方法。IDA可以促进一系列生物学/环境重要物种的检测,提供一种检测复杂分析物或确定和鉴别未知样品混合物的方法。这些属性通常是传统分子传感器无法实现的,基于反应的传感器/化学传感器。IDA的先驱Inouye,Shinkai和Anslyn启发了全世界的研究人员,以发展这一想法的各种扩展。自早期工作以来,指示剂位移分析的领域已扩展到包括:对映选择性指示剂位移分析(eIDA),荧光指示剂位移分析(FIDA),基于反应的指示剂位移分析(RIA),DimerDye拆卸分析(DDA),分子内指示剂位移测定(I​​IDA),变构指示剂位移测定(AIDA),机械控制的指示器位移测定(MC-IDA)和淬灭剂位移测定(QDA)。这些IDA的简单性,低成本,高灵敏度以及执行高通量自动化分析的能力((感应阵列)已导致其在分子感应中的广泛应用,以及其他常见方法,例如基于反应的传感器和化学传感器。在本文中,我们重点介绍了用于开发IDA的各种设计策略,包括新报告的这些系统扩展的设计策略。为实现这一目标,我们根据目标分析物,每种分析物的重要性以及报告的IDA系统将审查分为几部分。此外,每个部分都包含有关IDA的好处和每个系统的局限性的详细信息。在总结本教程总结时,我们重点介绍了与开发新IDA相关的当前挑战,并提出了未来的潜在研究途径。
更新日期:2020-12-09
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