Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)
Parallel Multiparameter Study of PEI-Functionalized Gold Nanoparticle Synthesis for Biomedical Applications: Part 2. Elucidating the Role of Surface Chemistry and Polymer Structure in Performance
Langmuir ( IF 3.9 ) Pub Date : 2020-11-10 , DOI: 10.1021/acs.langmuir.0c02630 Tae Joon Cho 1 , Justin M. Gorham 1 , John M. Pettibone 1 , Jingyu Liu 1 , Jiaojie Tan 2 , Vincent A. Hackley 1
Langmuir ( IF 3.9 ) Pub Date : 2020-11-10 , DOI: 10.1021/acs.langmuir.0c02630 Tae Joon Cho 1 , Justin M. Gorham 1 , John M. Pettibone 1 , Jingyu Liu 1 , Jiaojie Tan 2 , Vincent A. Hackley 1
Affiliation
Elucidating the polyethyleneimine (PEI) chemistry to predictively and reproducibly synthesize gold nanoparticle (AuNP)-PEI conjugates with desired properties has been elusive despite evaluation in numerous studies and reported enhanced properties. The lack of reproducible methods to control the core size and stability has led to contradictory results for performance and safety; thus, advancement of the conjugate platform for commercial use has likely been hindered. Recently, we reported a robust, reproducible method for synthesizing PEI-functionalized AuNPs (Au-PEIs), providing an opportunity to investigate structure–function relationships and to further investigate synthesis parameters affecting performance, where only materials stable in biological media are candidates for use. The properties of Au-PEIs prepared by the optimized reduction of HAuCl4 using four different structural variants of PEI changed significantly with the PEI molar mass and backbone form (branched or linear). In the present study using our previously reported synthesis procedure, comprehensive analysis of properties such as size distribution, surface plasmon resonance (SPR), morphological state, surface functionality, and the shelf life has been systematically evaluated to elucidate the role of surface chemistry and reactive groups involved in conjugation, as a function of conjugate size and morphology. Being important for commercial adoption, the chemistry was related to the observed colloidal stability of the product in relevant media, including exposure to physiological variables such as salt, pH, proteins, and thermal changes. Overall, this work advances progress toward smart design of engineered nanoscale drug delivery systems and devices by providing unreported details of contributions affecting formation, stability, and fate.
中文翻译:
用于生物医学应用的PEI功能化金纳米粒子合成的并行多参数研究:第2部分。阐明表面化学和聚合物结构在性能中的作用
尽管在许多研究中进行了评估并报告了增强的性能,但阐明聚乙烯亚胺(PEI)化学方法以可预测和可重复的方式合成具有所需性能的金纳米颗粒(AuNP)-PEI共轭物仍然难以捉摸。缺乏控制核心尺寸和稳定性的可重复方法,导致了性能和安全性上相互矛盾的结果。因此,用于商业用途的缀合物平台的发展可能受到阻碍。最近,我们报道了一种强大,可重现的方法,用于合成PEI功能化的AuNP(Au-PEI),为研究结构与功能之间的关系以及进一步研究影响性能的合成参数提供了机会,其中只有在生物介质中稳定的材料才可以使用。4使用PEI的四个不同结构变体会随着PEI摩尔质量和主链形式(支链或线性)而发生显着变化。在本研究中,使用我们先前报道的合成方法,已对诸如尺寸分布,表面等离振子共振(SPR),形态状态,表面功能性和保质期等性质的综合分析进行了系统评估,以阐明表面化学和反应性的作用。与缀合有关的基团是缀合物大小和形态的函数。对于商业应用而言,化学很重要,它与产品在相关介质中观察到的胶体稳定性有关,包括暴露于生理变量(如盐,pH,蛋白质和热变化)下。总体,
更新日期:2020-11-25
中文翻译:
用于生物医学应用的PEI功能化金纳米粒子合成的并行多参数研究:第2部分。阐明表面化学和聚合物结构在性能中的作用
尽管在许多研究中进行了评估并报告了增强的性能,但阐明聚乙烯亚胺(PEI)化学方法以可预测和可重复的方式合成具有所需性能的金纳米颗粒(AuNP)-PEI共轭物仍然难以捉摸。缺乏控制核心尺寸和稳定性的可重复方法,导致了性能和安全性上相互矛盾的结果。因此,用于商业用途的缀合物平台的发展可能受到阻碍。最近,我们报道了一种强大,可重现的方法,用于合成PEI功能化的AuNP(Au-PEI),为研究结构与功能之间的关系以及进一步研究影响性能的合成参数提供了机会,其中只有在生物介质中稳定的材料才可以使用。4使用PEI的四个不同结构变体会随着PEI摩尔质量和主链形式(支链或线性)而发生显着变化。在本研究中,使用我们先前报道的合成方法,已对诸如尺寸分布,表面等离振子共振(SPR),形态状态,表面功能性和保质期等性质的综合分析进行了系统评估,以阐明表面化学和反应性的作用。与缀合有关的基团是缀合物大小和形态的函数。对于商业应用而言,化学很重要,它与产品在相关介质中观察到的胶体稳定性有关,包括暴露于生理变量(如盐,pH,蛋白质和热变化)下。总体,