Lipids have been recently proposed as key molecules for virus entry and egress, and lipid biosynthesis and signaling were reported necessary for some viruses during replication and infection. The silkworm Bombyx mori is an important economic insect and a model organism, but its lipid profiles have not been systematically investigated. Most silkworm strains are susceptible to the B. mori nuclear polyhedrovirus (BmNPV), a baculovirus that causes serious loss to the sericulture industry. Previously, our lab has screened a natural mutant of B. mori that is highly resistant to BmNPV. In this study, a comprehensive lipidomic analysis by ultrahigh pressure liquid chromatography-mass spectrometry (UPLC-MS) was carried out on the BmNPV-susceptible strain 306 and resistant strain NB (data deposited in MetaboLight MTBLS2142). Comparisons of the lipid profiles between the two strains reveal that phosphosphingolipids, diacylglycerolipids, ceramides, and quinones were present at notably higher levels in the susceptible strain, while lysophosphocholines were found at a higher level in the resistant strain. BmNPV administration changed the lipid profiles in both strains, revealing key lipids involved in virus infection and immune response. Some key enzymes in the lipid biosynthesis pathway were analyzed for their activities in the two silkworm strains and their virus-administered counterparts, underlining the relation among lipid biosynthesis, viral resistance, and immune response in the host.

中文翻译：

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家蚕的血脂组图谱：病毒感染的影响。

最近有人提出脂质是病毒进入和排出的关键分子，并且据报道脂质的合成和信号转导对于某些病毒在复制和感染过程中是必需的。蚕家蚕是重要的经济昆虫和生物模型，但其血脂没有得到系统的研究。大多数蚕株对桑蚕核多角体病毒（BmNPV）敏感，杆状病毒对蚕桑业造成严重损失。以前，我们的实验室已经筛选出了B. mori的天然突变体对BmNPV高度耐药。在这项研究中，对BmNPV易感菌株306和抗性菌株NB进行了超高压液相色谱-质谱（UPLC-MS）的全面脂质组分析（数据保存在MetaboLight MTBLS2142中）。两种菌株之间脂质谱的比较表明，在易感菌株中磷酸鞘脂，二酰基甘油脂，神经酰胺和醌的含量明显较高，而在抗性菌株中发现溶血卵胆碱的含量较高。BmNPV施用改变了这两种菌株的脂质谱，揭示了参与病毒感染和免疫反应的关键脂质。分析了脂质生物合成途径中的一些关键酶在这两种家蚕菌株中以及在其病毒管理的对应物中的活性，