Nanofibrous poly(l-lactic acid) (PLLA) membrane-simulated extracellular matrices (ECMs) can be used in the biomedical field. However, the hydrophobic nature and poor osteoinductive property of PLLA limit its application in guided bone regeneration (GBR). In this work, a methacrylated gelatin/nano-HA (GelMA/nHA) complex was first synthesized in situ and then introduced into PLLA to fabricate biomimetic GelMA/nHA/PLLA membranes, mimicking the nanofibrous architecture and composition of ECMs by electrospinning and photocrosslinking. Compared to PLLA and GelMA/PLLA membranes, the novel GelMA/nHA/PLLA membranes demonstrated better tensile, hydrophilic, water sorption, and degradation properties. An in vitro biological evaluation indicated that the membranes promoted human bone marrow-derived mesenchymal stem cell (hBMSC) proliferation, adhesion, and osteogenic differentiation. Critical-sized defects in rat models were used to evaluate the bone regeneration performances of the three kinds of membranes in vivo, and the GelMA/nHA/PLLA membranes demonstrated excellent osteogenic regeneration potential. Therefore, GelMA/nHA/PLLA membranes have wide application prospects in bioengineering applications such as GBR treatment.

中文翻译：

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甲基丙烯酸明胶/纳米羟基磷灰石/聚（1-乳酸）的仿生膜增强骨骼再生

纳米纤维聚（升（乳酸）（PLLA）膜模拟的细胞外基质（ECM）可以用于生物医学领域。但是，PLLA的疏水性和不良的骨诱导特性限制了其在引导性骨再生（GBR）中的应用。在这项工作中，首先就地合成了甲基丙烯酸明胶/纳米HA（GelMA / nHA）复合物，然后将其引入PLLA中以制备仿生的GelMA / nHA / PLLA膜，通过电纺丝和光交联模拟了ECM的纳米纤维结构和组成。与PLLA和GelMA / PLLA膜相比，新型GelMA / nHA / PLLA膜具有更好的拉伸，亲水，吸水和降解性能。体外生物学评估表明，该膜可促进人骨髓来源的间充质干细胞（hBMSC）增殖，粘附，和成骨分化。大鼠模型中的临界大小缺陷用于评估三种膜在体内的骨再生性能，而GelMA / nHA / PLLA膜显示出极好的成骨再生潜力。因此，GelMA / nHA / PLLA膜在生物工程应用（如GBR处理）中具有广阔的应用前景。