Ribozymes and deoxyribozymes are catalytic RNA and DNA, respectively, that catalyze chemical reactions such as self-cleavage or ligation reactions. While some ribozymes are found in nature, a larger variety of ribozymes and deoxyribozymes have been discovered by in vitro selection from random sequences. These catalytic nucleic acids, especially ribozymes, are of fundamental interest because they are crucial for the RNA world hypothesis, which suggests that RNA played a central role in both the propagation of genetic information and catalyzing metabolic reactions in primordial life prior to the emergence of proteins and DNA. On the practical side, catalytic nucleic acids have been extensively engineered for various applications, such as biosensors and genetic devices for synthetic biology. Therefore, it is important to gain a deeper understanding of the sequence–function relationships of ribozymes and deoxyribozymes.

中文翻译：

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测序的核糖核酸和脱氧核糖核酸高通量分析与工程

核酶和脱氧核酶分别是催化RNA和DNA，其催化化学反应，例如自切割或连接反应。尽管在自然界中发现了一些核酶，但通过从随机序列中进行体外选择，已经发现了更多种核酶和脱氧核酶。这些催化核酸，尤其是核酶，是至关重要的，因为它们对于RNA世界假说至关重要，这表明RNA在蛋白质出现之前的原始生命中，在遗传信息的传播和催化代谢反应中都起着核心作用。和DNA。在实践方面，催化核酸已被广泛设计用于各种应用，例如生物传感器和用于合成生物学的遗传装置。因此，