Abstract

Low concentrations of biomarkers as well as the complexity of biological samples make the clinical diagnoses of several diseases a challenging task. Sample preparation protocols remain a fundamental piece in the puzzle of analytical processes, and smart sorbents including molecularly imprinted polymers (MIPs) have been successfully used in this case. In this review, we depict the state of art for the rational design of MIPs to be used in solid phase extraction of disease biomarkers from biological samples. The topics are divided into (1) strategies for MIP syntheses, (2) setups for sample preparation protocols with MIPs, (3) the applications of these combined principles in the analyses of different classes of disease biomarkers, and (4) remaining challenges and future trends for the application of Molecular Imprinting Technology in sample preparation for clinical diagnosis.

摘要

低浓度的生物标志物以及生物样本的复杂性使得几种疾病的临床诊断成为一项具有挑战性的任务。样品制备协议仍然是分析过程难题中的基本组成部分，包括分子印迹聚合物 (MIP) 在内的智能吸附剂已成功用于此案例。在这篇综述中，我们描述了用于从生物样品中固相提取疾病生物标志物的 MIP 的合理设计的最新技术。主题分为 (1) MIP 合成策略，(2) MIP 样品制备方案的设置，(3) 这些组合原理在不同类别疾病生物标志物分析中的应用，