Bisimidazolium receptors, tagged with chromophoric pyrene at one end and linked to an n-alkyl chain at the other, underwent self-assembly in aqueous media depending on the length of the alkyl segment. The amphiphilic derivatives having n-decyl or longer chains, formed nano-assemblies with cyanic–green emission resulting from the stacked pyrene chromophores in the aggregates. The presence of positive surface charges on the multivalent aggregates led to ATP binding which was accompanied by a significant increase in the excimeric emission intensity. This provided a convenient way of monitoring ATP binding in a “turn-on” mode and an efficient detection of ATP was achieved in aqueous buffer and also in buffer containing 150 mM NaCl at physiological pH value. Furthermore, the multivalent aggregates demonstrated a significant selectivity in ATP detection over ADP, AMP and pyrophosphate.

中文翻译：

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通过“开启”荧光反应，通过双咪唑两亲物的多价纳米组装选择性地识别ATP

在末端用发色pyr标记并在另一端连接到正烷基链的双咪唑鎓受体在水性介质中根据烷基链段的长度进行自组装。具有n的两亲衍生物-癸基或更长链形成纳米聚集体，该聚集体由聚集体中堆积的pyr发色团产生，呈氰绿色发射。多价聚集体上存在正表面电荷会导致ATP结合，并伴随激子发射强度的显着增加。这提供了一种以“开启”模式监测ATP结合的简便方法，并且在水性缓冲液中以及生理pH值下包含150 mM NaCl的缓冲液中都实现了ATP的有效检测。此外，与ADP，AMP和焦磷酸盐相比，多价聚集体在ATP检测中表现出显着的选择性。