Platelet-rich plasma (PRP) has seen wide clinical use owing to its regenerative and repair abilities. OBJECTIVE To investigate anti-photoaging effects of pre- and post-treatment of PRP on UVB-damaged HaCaT cells. METHODS HaCaT cells were irradiated with 80 mJ/cm2 UVB, before or after PRP treatment (1000×107 /L) and following measurements were taken: Survival rate of UVB-irradiated HaCaT cells, malondialdehyde (MDA) content, and activities of glutathione peroxidase (GSH-Px), superoxide dismutase (SOD), catalase (CAT). Western blot was used to determine the effect of different PRP intervention on the expression of PI3K, AKT, ERK, MMP-1, MMP-9, TIMP-1 and γ-H2AX in the UVB-irradiated HaCaT cells. RESULTS Pre- and Post-PRP treatment reduced MDA content and increased the activities of GSH-Px, SOD, and CAT in photoaged HaCaT cells. These changes resulted in reduced cytotoxic effects. Besides, different PRP intervention promoted cell proliferation via PI3K/AKT pathway. Furthermore, PRP application suppressed the expression of γ-H2AX. Also, PRP intervention alleviated photoaging effects by upregulating the expression level of tissue inhibitor of metalloproteinases-1(TIMP-1) while downregulating matrix metalloproteinase(MMP) expression level in photoaged HaCaT cells. CONCLUSION Pre- and Post-PRP treatment play anti-photoaging role through strengthening cellular oxidative defense capacity, mitigating MMP expression, alleviating DNA damages and promoting proliferation of UVB-irradiated HaCaT cells.

中文翻译：

---

富血小板血浆前后处理对 UVB 损伤 HaCaT 角质形成细胞的抗光老化作用

富血小板血浆 (PRP) 因其再生和修复能力而在临床上得到广泛应用。目的探讨PRP前后处理对UVB损伤HaCaT细胞的抗光老化作用。方法 HaCaT 细胞在 PRP 处理之前或之后（1000×107 /L）用 80 mJ/cm2 UVB 照射，并进行以下测量：UVB 照射的 HaCaT 细胞的存活率、丙二醛（MDA）含量和谷胱甘肽过氧化物酶的活性(GSH-Px)、超氧化物歧化酶 (SOD)、过氧化氢酶 (CAT)。Western印迹用于确定不同PRP干预对UVB照射的HaCaT细胞中PI3K、AKT、ERK、MMP-1、MMP-9、TIMP-1和γ-H2AX表达的影响。结果 PRP 前后处理降低了 MDA 含量，增加了光老化 HaCaT 细胞中 GSH-Px、SOD 和 CAT 的活性。这些变化导致细胞毒性作用降低。此外，不同的 PRP 干预通过 PI3K/AKT 通路促进细胞增殖。此外，PRP 应用抑制了 γ-H2AX 的表达。此外，PRP 干预通过上调组织金属蛋白酶组织抑制剂 1 (TIMP-1) 的表达水平同时下调光老化 HaCaT 细胞中基质金属蛋白酶 (MMP) 的表达水平来减轻光老化效应。结论 PRP前后处理通过增强细胞氧化防御能力、减轻MMP表达、减轻DNA损伤和促进UVB照射的HaCaT细胞增殖而发挥抗光老化作用。此外，PRP 干预通过上调组织金属蛋白酶组织抑制剂 1 (TIMP-1) 的表达水平同时下调光老化 HaCaT 细胞中基质金属蛋白酶 (MMP) 的表达水平来减轻光老化效应。结论 PRP前后处理通过增强细胞氧化防御能力、减轻MMP表达、减轻DNA损伤和促进UVB照射的HaCaT细胞增殖而发挥抗光老化作用。此外，PRP 干预通过上调组织金属蛋白酶组织抑制剂 1 (TIMP-1) 的表达水平同时下调光老化 HaCaT 细胞中基质金属蛋白酶 (MMP) 的表达水平来减轻光老化效应。结论 PRP前后处理通过增强细胞氧化防御能力、减轻MMP表达、减轻DNA损伤和促进UVB照射的HaCaT细胞增殖而发挥抗光老化作用。