In “Borrelia burgdorferi outer surface protein C (OspC) binds complement component C4b and confers bloodstream survival”, by Caine et al. (2017)which was published in volume 19, issue 12, September 2017, the authors wish to notify readers that we have not been able to reliably reproduce the data in Figure 5. The corresponding text “To determine if OspC is able to directly inhibit the complement cascade allowing WT B. burgdorferi to survive in the bloodstream of mice, we performed an ELISA‐based complement activation assay. Plates were coated with initiators of each pathway of the complement cascade; Immunoglobulin M (IgM; classical pathway), mannan (lectin pathway), lipopolysaccharide (LPS; alternative pathway), or buffer (negative control). Coated plates were incubated with a mixture of pooled active human serum and various concentrations of purified GST‐OspC B31, GST, or buffer, and complement deposition was measured in each well by ELISA (Figure 5). Wells coated with IgM or mannan and incubated with 1000 nM GST‐ OspC have significantly lower levels of deposited complement compared to wells incubated in GST (Figure 5a,b). This effect is not seen in LPS coated wells, where the addition of GST‐OspC does not inhibit complement deposition compared to GST at any concentration tested (Figure 5c) and Figure 5” should be removed from the paper. In addition, Experimental Procedures section 4.13 “Complement Inhibition ELISAs” should be removed.

All other data shown are reproducible, so our primary conclusion is supported.

Caine等人在“ Borrelia burgdorferi外表面蛋白C（OspC）结合补体成分C4b并赋予血流存活率”中。（2017年）出版于2017年9月第19卷第19期，作者希望通知读者我们无法可靠地重现图5中的数据。相应的文本“确定OspC是否能够直接抑制补体级联，允许WT B. burgdorferi为了在小鼠血流中存活，我们进行了基于ELISA的补体激活测定。用补体级联的每个途径的引发剂涂覆板；免疫球蛋白M（IgM；经典途径），甘露聚糖（凝集素途径），脂多糖（LPS；替代途径）或缓冲液（阴性对照）。将包被的板与混合的活性人血清和各种浓度的纯化的GST-OspC B31，GST或缓冲液的混合物一起孵育，并通过ELISA测量每个孔中的补体沉积（图5）。与在GST中孵育的孔相比，涂有IgM或甘露聚糖并与1000 nM GST-OspC孵育的孔的沉积补体水平显着降低（图5a，b）。在LPS涂层的孔中看不到这种效果，在任何测试浓度下，与GST相比，添加GST-OspC都不会抑制补体沉积（图5c），应从纸上取下“图5”。此外，应删除实验程序第4.13节“补体抑制ELISA”。

显示的所有其他数据都是可重复的，因此我们的主要结论得到了支持。