Nucleic acids are relevant biopolymers in therapy and diagnosis, for which their purity and biological activity are of crucial relevance. However, these features are difficult to achieve by cost-effective methods. Herein, we report the functionalization of a macroporous chromatographic support functionalized with an ionic liquid (IL) with remarkable performance to purify nucleic acids. An initial screening with distinct IL chemical structures supported in silica was carried out, allowing to identify the IL 1-methyl-3-propylimidazolium chloride as the most promising ligand. A chromatographic macroporous matrix able to be used in preparative liquid chromatography was then functionalized and binding/elution studies were performed. The IL 1-methyl-3-propylimidazolium chloride acts as a multimodal ligand with a remarkable dynamic binding capacity. This macroporous support allows the (one-step) purification of nucleic acids, namely small RNAs, ribosomal RNA, and genomic DNA, from a bacterial lysate, and can be regenerated and reused without compromising its separation performance.

核酸是治疗和诊断中相关的生物聚合物，对其纯度和生物活性至关重要。但是，这些功能很难通过经济有效的方法来实现。在本文中，我们报道了用离子液体（IL）进行功能化的大孔色谱支持物的功能化，其具有卓越的纯化核酸的性能。进行了在二氧化硅中负载的具有独特IL化学结构的初步筛选，从而可以确定IL 1-甲基-3-丙基咪唑鎓氯化物是最有希望的配体。然后将能够用于制备型液相色谱的色谱大孔基质功能化，并进行结合/洗脱研究。IL 1-甲基-3-丙基咪唑鎓氯化物充当具有显着动态结合能力的多峰配体。