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The neuroprotective effect of ethanolic extract Ocimum sanctum Linn. in the regulation of neuronal density in hippocampus areas as a central autobiography memory on the rat model of Alzheimer’s disease
Journal of Chemical Neuroanatomy ( IF 2.8 ) Pub Date : 2021-01-01 , DOI: 10.1016/j.jchemneu.2020.101885
Made Bagus Auriva Mataram 1 , Puspa Hening 1 , Fitria N Harjanti 2 , Srikanth Karnati 3 , Widya Wasityastuti 4 , Dwi Aris Agung Nugrahaningsih 5 , Dwi Liliek Kusindarta 2 , Hevi Wihadmadyatami 2
Affiliation  

The aim of this study was to identify the effects of Ocimum sanctum Linn. ethanolic extract (OSE) on the neurons of the CA1, CA3, and DG hippocampal areas with the use of in vivo and in vitro models of Alzheimer's diseases (AD). Twenty-one two-month-old male rats were divided into three groups: untreated (Group A, n = 3), AD rats model pretreated with OSE followed by induction for Trimethyltin (TMT) on day 7 (group B, n = 9), and AD rats model treated with OSE both as pre-TMT introduction for 7 days and post-TMT induction for 21 days (group C, n = 9). AD rats were sacrificed on days 7, 14, and 21, and brain samples were collected and analyzed for neuronal density and neuropeptide Y (NPY) immunoreactivity. To corroborate the in vivo observations, HEK-293 cells were treated with TMT and used as an in vitro model of AD. The results were then analyzed using FITC Annexin V and flow cytometry. Nuclear fragmentation was observed in cells stained with Hoechst 33342 by confocal microscopy. The results showed a significant increase in the number of neurons and NPY expression in the AD rats that were pre- and post-treated with OSE (p < 0.05). Indeed, OSE was able to retain and promote neuronal density in the rat model of AD. Further studies of an in vitro model of neurodegeneration with Ocimum sanctum Linn. ethanolic extract inhibited apoptosis in TMT-induced HEK-293 cells. Moreover, OSE prevented nuclear fragmentation, which was confirmed by staining the nuclei of HEK-293 cells. Taken together, there findings suggest that OSE has the potential as a neuroprotective agent (retaining the autobiographical memory),and the neuroproliferation of neurons in the CA1, CA3, and DG hippocampal areas in the rats¡ model of neurodegeneration was mediated by activation of NPY expression.

中文翻译:

乙醇提取物 Ocimum sanctum Linn 的神经保护作用。海马区神经元密度的调节作为阿尔茨海默病大鼠模型的中枢自传记忆

本研究的目的是确定 Ocimum sanctum Linn 的作用。乙醇提取物 (OSE) 在 CA1、CA3 和 DG 海马区的神经元上使用阿尔茨海默病 (AD) 的体内和体外模型。将 21 只 2 个月大的雄性大鼠分为三组:未治疗组(A 组,n = 3),用 OSE 预处理然后在第 7 天诱导三甲基锡(TMT)的 AD 大鼠模型(B 组,n = 9 ),和用 OSE 治疗的 AD 大鼠模型作为 TMT 前引入 7 天和 TMT 诱导后 21 天(C 组,n = 9)。在第 7、14 和 21 天处死 AD 大鼠,收集脑样本并分析神经元密度和神经肽 Y (NPY) 免疫反应性。为了证实体内观察,HEK-293 细胞用 TMT 处理并用作 AD 的体外模型。然后使用 FITC Annexin V 和流式细胞术分析结果。通过共聚焦显微镜在用 Hoechst 33342 染色的细胞中观察到核碎裂。结果显示,在用 OSE 预处理和后处理的 AD 大鼠中,神经元数量和 NPY 表达显着增加(p < 0.05)。事实上,OSE 能够保留和促进 AD 大鼠模型中的神经元密度。用 Ocimum sanctum Linn 进一步研究神经变性的体外模型。乙醇提取物抑制 TMT 诱导的 HEK-293 细胞凋亡。此外,OSE 可防止核碎裂,这通过染色 HEK-293 细胞的细胞核得到证实。综上所述,研究结果表明 OSE 具有作为神经保护剂(保留自传记忆)的潜力,以及 CA1、CA3、
更新日期:2021-01-01
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