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Severity assessment in the Nicotiana tabacum-Xylella fastidiosa subsp. pauca pathosystem: design and interlaboratory validation of a standard area diagram set
Tropical Plant Pathology ( IF 2.5 ) Pub Date : 2020-11-09 , DOI: 10.1007/s40858-020-00401-5
Willian Eduardo Lino Pereira , Sheila Maria Pereira de Andrade , Emerson M. Del Ponte , Mariana Bossi Esteves , Maria Cristina Canale , Marco Aurélio Takita , Helvécio Della Coletta-Filho , Alessandra Alves De Souza

Though an experimental host of Xylella fastidiosa, tobacco (Nicotiana tabacum) is an excellent plant model for biological and functional genomics studies of host-pathogen interactions involving X. fastidiosa. Symptoms induced by X. fastidiosa subsp. pauca on tobacco have been characterized, but severity assessment relies on an ordinal scale developed for symptoms on citrus. We designed a standard area diagram set (SADs) to aid in the visual estimation of percent area affected (% severity) and performed a multi-laboratory validation on tobacco cv. Havana, inoculated with X. fastidiosa subsp. pauca. Inoculated plants were monitored over time and digital images of the symptoms recorded. Three different software programs (APS Asses, ImageJ, and Leaf Doctor) were used to segment the images and calculate percent severity. Ten true-color images composed a 10-image SADs (0.5, 5, 10, 15, 25, 35, 45, 55, 65, and 75%). Fifty raters at four research groups (laboratories) assigned percent severity first without and then with the SADs on the same testing image set of 40 images following the same instructions provided by an examiner at each laboratory. The means of percent severity by all software programs were assumed to represent the actual percent severity given the perfect agreement between them. The unaided estimates were less precise, biased towards overestimation (up to 50% points) and less concordant among raters. Accuracy and precision varied considerably among the raters, and the effect of the SADs on the overall concordance to the actual severity was dependent on the laboratory; in all but one laboratory, the group means of the agreement index statistically improved. The between-rater agreement improved in all laboratories when using the SADs. The SADs may help to standardize and allow comparison across different laboratories, but care should be taken during instructions on how to discriminate symptoms that are not readily discernible as well as on how to correctly use the SADs.

中文翻译:

Nicotiana tabacum-Xylella fastidiosa subsp. 的严重程度评估。pauca 病理系统:标准面积图集的设计和实验室间验证

虽然烟草(Nicotiana tabacum)是苛养木霉的实验宿主,但它是一种极好的植物模型,可用于涉及苛养木霉的宿主-病原体相互作用的生物学和功能基因组学研究。由 X. fastidiosa subsp. 引起的症状。烟草上的 pauca 已被表征,但严重性评估依赖于针对柑橘症状制定的顺序量表。我们设计了一个标准面积图集 (SAD) 以帮助直观估计受影响的面积百分比(严重性百分比),并对烟草 cv 进行了多实验室验证。哈瓦那,接种 X. fastidiosa subsp。保卡。随着时间的推移监测接种的植物并记录症状的数字图像。三个不同的软件程序(APS Asses、ImageJ 和 Leaf Doctor)用于分割图像并计算严重程度百分比。10 个真彩色图像组成了 10 个图像 SAD(0.5、5、10、15、25、35、45、55、65 和 75%)。四个研究组(实验室)的 50 名评估员按照每个实验室的检查员提供的相同说明,在 40 幅图像的相同测试图像集上首先分配严重程度百分比,然后分配 SAD。假定所有软件程序的严重程度百分比平均值代表它们之间完全一致的实际百分比严重程度。独立估计不太准确,偏向于高估(高达 50% 的点),并且评分者之间的一致性较差。评估者之间的准确度和精密度差异很大,SAD 对实际严重程度整体一致性的影响取决于实验室;除了一个实验室之外的所有地方,一致性指数的组均值在统计上有所改善。使用 SAD 时,所有实验室的评估者之间的一致性都得到了改善。SAD 可能有助于标准化并允许在不同实验室之间进行比较,但在说明如何区分不易辨别的症状以及如何正确使用 SAD 时应小心谨慎。
更新日期:2020-11-09
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