The current study investigated the change in umbilical cord tissue and the number of markers of Wharton’s jelly mesenchymal stem cells (WJ-MSC) in pregnant women with gestational diabetes (GDM), with chronic diabetes who developed nephropathy as vascular complication (VC-PGDM), and healthy pregnant women as the control. The umbilical cords (UC) were investigated by the histomorphological method and the number of WJ-MSC were detected by flow-cytometry using the CD90, CD44, CD105, and CD73 markers in Wharton’s jelly (WJ) isolated from fresh umbilical cords. The number of positive cells for CD 90, CD44, CD105, and CD73 were found to be elevated in the GDM group, whereas it was significantly diminished in the VC-PGDM group (p = 0.001, p = 0.001, p = 0.001, and p = 0.001). The only histopathological sign in the GDM group were an increased number of pores in the Wharton jelly. Artery wall thickness/cord diamater ratio was increased, which indicates an increase of the artery wall thickness in the VC- PGDM group (p = 0.039 and p = 0.048). The increase in umbilical cord diameter and number of Wharton jelly mesenchymal stem cells in babies of gestational diabetic mothers was considered as an effect of macrosomia seen in babies of mothers with gestational diabetes. Vasculopathy, a long-term complication of diabetes, is known to affect all tissues by causing marked lower perfusion and hypoxia, as well as a decrease in the MSC number in our study.

当前的研究调查了妊娠糖尿病（GDM），患有肾病的血管并发症（VC-PGDM）的孕妇的脐带组织变化和沃顿胶质间充质干细胞（WJ-MSC）的数量，并以健康孕妇为对照。使用组织形态学方法研究脐带（UC），并使用流式细胞仪检测WJ-MSC的数量，其中使用了从新鲜脐带中分离出的沃顿胶冻（WJ）中的CD90，CD44，CD105和CD73标记。在GDM组中发现CD 90，CD44，CD105和CD73的阳性细胞数量增加，而在VC-PGDM组中则明显减少（p = 0.001，p = 0.001，p = 0.001和p = 0.001）。GDM组中唯一的组织病理学迹象是沃顿胶冻中毛孔数量增加。动脉壁厚度/脐带直径比例增加，这表明VC-PGDM组的动脉壁厚度增加（p = 0.039和p = 0.048）。妊娠糖尿病母亲婴儿的脐带直径和沃顿果冻间充质干细胞数量的增加被认为是妊娠糖尿病母亲婴儿所见的巨大儿的影响。血管病是糖尿病的一种长期并发症，在我们的研究中，血管病通过引起明显的较低的灌注和缺氧以及MSC数量的减少而影响所有组织。039，p = 0.048）。妊娠糖尿病母亲婴儿的脐带直径和沃顿果冻间充质干细胞数量的增加被认为是妊娠糖尿病母亲婴儿所见的巨大儿的影响。血管病是糖尿病的一种长期并发症，在我们的研究中，血管病通过引起明显的较低的灌注和缺氧以及MSC数量的减少而影响所有组织。039，p = 0.048）。妊娠糖尿病母亲婴儿的脐带直径和沃顿果冻间充质干细胞数量的增加被认为是妊娠糖尿病母亲婴儿所见的巨大儿的影响。血管病是糖尿病的一种长期并发症，在我们的研究中，血管病通过引起明显的较低的灌注和缺氧以及MSC数量的减少而影响所有组织。