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Renal proximal tubular epithelial cells: review of isolation, characterization, and culturing techniques
Molecular Biology Reports ( IF 2.8 ) Pub Date : 2020-11-10 , DOI: 10.1007/s11033-020-05977-4
Matic Mihevc 1 , Tadej Petreski 1, 2 , Uroš Maver 2, 3 , Sebastjan Bevc 1, 3
Affiliation  

The kidney is a complex organ, comprised primarily of glomerular, tubular, mesangial, and endothelial cells, and podocytes. The fact that renal cells are terminally differentiated at 34 weeks of gestation is the main obstacle in regeneration and treatment of acute kidney injury or chronic kidney disease. Furthermore, the number of chronic kidney disease patients is ever increasing and with it the medical community should aim to improve existing and develop new methods of renal replacement therapy. On the other hand, as polypharmacy is on the rise, thought should be given into developing new ways of testing drug safety. A possible way to tackle these issues is with isolation and culture of renal cells. Several protocols are currently described to isolate the desired cells, of which the most isolated are the proximal tubular epithelial cells. They play a major role in water homeostasis, acid–base control, reabsorption of compounds, and secretion of xenobiotics and endogenous metabolites. When exposed to ischemic, toxic, septic, or obstructive conditions their death results in what we clinically perceive as acute kidney injury. Additionally, due to renal cells' limited regenerative potential, the profibrotic environment inevitably leads to chronic kidney disease. In this review we will focus on human proximal tubular epithelial cells. We will cover human kidney culture models, cell sources, isolation, culture, immortalization, and characterization subdivided into morphological, phenotypical, and functional characterization.



中文翻译:

肾近端肾小管上皮细胞:分离,表征和培养技术的审查。

肾脏是一个复杂的器官,主要由肾小球,肾小管,肾小球膜和内皮细胞以及足细胞组成。妊娠34周时肾细胞终末分化的事实是急性肾损伤或慢性肾脏疾病的再生和治疗的主要障碍。此外,慢性肾脏病患者的数量不断增加,医学界应以此为目标,以改善现有的肾脏替代疗法并开发新的方法。另一方面,随着多元药学的兴起,应该考虑开发新的测试药物安全性的方法。解决这些问题的一种可能方法是分离和培养肾细胞。当前描述了几种方案以分离所需的细胞,其中最分离的是近端肾小管上皮细胞。它们在水体内平衡,酸碱控制,化合物的重吸收以及异生物素和内源性代谢物的分泌中起主要作用。当暴露于缺血,毒性,败血性或阻塞性疾病时,它们的死亡导致我们在临床上将其视为急性肾损伤。另外,由于肾细胞的再生潜力有限,纤维化环境不可避免地导致慢性肾脏疾病。在这篇综述中,我们将重点关注人类近端肾小管上皮细胞。我们将涵盖人类肾脏培养模型,细胞来源,分离,培养,永生化和表征,这些表征又细分为形态,表型和功能表征。当暴露于缺血,毒性,败血性或阻塞性疾病时,它们的死亡导致我们在临床上将其视为急性肾损伤。另外,由于肾细胞的再生潜力有限,纤维化环境不可避免地导致慢性肾脏疾病。在这篇综述中,我们将重点关注人类近端肾小管上皮细胞。我们将涵盖人类肾脏培养模型,细胞来源,分离,培养,永生化和表征,这些表征又细分为形态,表型和功能表征。当暴露于缺血,毒性,败血性或阻塞性疾病时,它们的死亡导致我们在临床上将其视为急性肾损伤。另外,由于肾细胞的再生潜力有限,纤维化环境不可避免地导致慢性肾脏疾病。在这篇综述中,我们将重点关注人类近端肾小管上皮细胞。我们将涵盖人类肾脏培养模型,细胞来源,分离,培养,永生化和表征,这些表征又细分为形态,表型和功能表征。在这篇综述中,我们将重点关注人类近端肾小管上皮细胞。我们将涵盖人类肾脏培养模型,细胞来源,分离,培养,永生化和表征,这些表征又细分为形态,表型和功能表征。在这篇综述中,我们将重点关注人类近端肾小管上皮细胞。我们将涵盖人类肾脏培养模型,细胞来源,分离,培养,永生化和表征,这些表征又细分为形态,表型和功能表征。

更新日期:2020-11-12
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