Spanish mackerel S. commerson belonging to family Scombridae, represent a group of highly commercial marine fisheries with an ever-growing demand world over. Analysing the genetic diversity of this species is of utmost importance and necessary for conservation purposes. Microsatellites are molecular tools with advantages that are ideal for population analyses. This study provides the first multiplex panel set of species-specific microsatellite loci for S. commerson that can be applied when assessing both intra- and inter population genetic variation. Microsatellite marker panels were developed in S. commerson, using Third Generation Sequencing technology in PacBio RSII, based on Single-Molecule Real-Time (SMRT). Thirty- two microsatellite loci were isolated and characterized for S. commerson, by genotyping 20 individuals each obtained from the Kochi and Veraval in the Arabian sea and Chennai along Bay of Bengal coast (n = 3). The number of alleles per locus in S. commerson varied from 4 to 17, while the mean observed and expected heterozygosities ranged from 0.656 to 0.753. The Polymorphic Information Content (PIC) were highly informative, 85% loci with PIC value 0 > 0.75. This suite of markers provides the first species specific nuclear multiplex microsatellite marker panels (32 loci) for S. commerson and thus allows assessment of different populations structures of the species across its distribution range, with more specificity. These newly developed loci have also been validated for cross transferability in another scomberid fish Scomberomorus guttatus.

属于S科的鱼（S. commerson）代表着一组高度商业化的海洋渔业，其需求在世界范围内不断增长。为了保护目的，分析该物种的遗传多样性至关重要。微卫星是分子工具，具有非常适合人口分析的优势。这项研究为沙门氏菌提供了物种多样性微卫星基因座的第一个多重面板组，可用于评估种群内和种群间的遗传变异。微卫星标记面板是使用单分子实时（SMRT）技术在PacBio RSII中使用第三代测序技术在S. commerson中开发的。通过对20个个体进行基因分型，分离了32个微卫星基因座，并对其进行了S. commerson鉴定，这些个体分别来自阿拉伯海和沿孟加拉湾沿岸的金奈的Kochi和Veraval（n = 3）。每个位点的等位基因的数量S. commerson改变从4到17，而观察到的平均和期望杂范围从0.656至0.753。多态信息含量（PIC）的信息量很高，有85％的基因座，PIC值0> 0.75。这套标记为S. commerson提供了第一个物种特异性的核多重微卫星标记板（32个基因座）因此，可以更广泛地评估其分布范围内物种的不同种群结构。这些新近形成的基因座也已在另一头苦fish鱼Scomberomorus guttatus中被证实具有交叉转移能力。