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Principal component analysis approach for comprehensive screening of salt stress-tolerant tomato germplasm at the seedling stage
Journal of Biosciences ( IF 2.9 ) Pub Date : 2020-11-11 , DOI: 10.1007/s12038-020-00111-9
J Sivakumar , John Elia P Prashanth , N Rajesh , Sridhar M Reddy , Osman Basha Pinjari

Salt stress is a major abiotic factor that affects the growth and yield of crops. The present study was carried out to assess the salt tolerance among the Arka Samrat, Arka Rakshak, YVU-1, S-22, YVU-2, and PKM-OP tomato germplasms using principal component analysis (PCA). Different salt (NaCl) concentrations like control, 0.04 M, 0.12 M, and 0.20 M were selected in order to classify them into sensitive and tolerant tomato germplasms based on 13 parameters. A significant variation was observed among the selected tomato germplasms towards salinity tolerance at the seedling stage. Shoot length, root length, fresh weight, and dry weight parameters of the seedlings were decreased linearly with an increase in the external NaCl concentration. Salinization of plants has shown to reduce K + content and increase in the Na + accumulation, Ca 2+ , and Catalase activity. Salt stress also increased electrolyte leakage and reduced relative water content of all germplasms. The maximum parameters were less affected in Arka Rakshak and Arka Samrat compared to the remaining germplasms at higher salt stress. The PCA analysis of 13 morphological and physiological variables indicated that Arka Rakshak and Arka Samrat germplasms were salt-tolerant and PKM-OP was susceptible. Thus PCA analysis results are useful for the identification of resistance and sensitive germplasms at the seedling stage.

中文翻译:

番茄苗期耐盐种质综合筛选主成分分析方法

盐胁迫是影响作物生长和产量的主要非生物因素。本研究旨在使用主成分分析 (PCA) 评估 Arka Samrat、Arka Rakshak、YVU-1、S-22、YVU-2 和 PKM-OP 番茄种质的耐盐性。选择不同的盐 (NaCl) 浓度,如对照、0.04 M、0.12 M 和 0.20 M,基于 13 个参数将它们分类为敏感和耐受番茄种质。在苗期,在选定的番茄种质中观察到显着的耐盐性差异。幼苗的枝长、根长、鲜重和干重参数随着外部 NaCl 浓度的增加而线性下降。植物的盐渍化已显示降低 K + 含量并增加 Na + 积累、Ca 2+ 、和过氧化氢酶活性。盐胁迫还增加了电解质泄漏并降低了所有种质的相对含水量。与在较高盐胁迫下的其余种质相比,Arka Rakshak 和 Arka Samrat 的最大参数受到的影响较小。13个形态和生理变量的PCA分析表明Arka Rakshak和Arka Samrat种质耐盐,PKM-OP易感。因此,PCA 分析结果可用于鉴定苗期抗性和敏感种质。13个形态和生理变量的PCA分析表明Arka Rakshak和Arka Samrat种质耐盐,PKM-OP易感。因此,PCA 分析结果可用于鉴定苗期抗性和敏感种质。13个形态和生理变量的PCA分析表明Arka Rakshak和Arka Samrat种质耐盐,PKM-OP易感。因此,PCA 分析结果可用于鉴定苗期抗性和敏感种质。
更新日期:2020-11-11
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