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Invited review: human air-liquid-interface organotypic airway tissue models derived from primary tracheobronchial epithelial cells—overview and perspectives
In Vitro Cellular & Developmental Biology - Animal ( IF 2.1 ) Pub Date : 2020-11-11 , DOI: 10.1007/s11626-020-00517-7
Xuefei Cao 1 , Jayme P Coyle 2 , Rui Xiong 1 , Yiying Wang 1 , Robert H Heflich 1 , Baiping Ren 1 , William M Gwinn 3 , Patrick Hayden 4 , Liying Rojanasakul 2
Affiliation  

The lung is an organ that is directly exposed to the external environment. Given the large surface area and extensive ventilation of the lung, it is prone to exposure to airborne substances, such as pathogens, allergens, chemicals, and particulate matter. Highly elaborate and effective mechanisms have evolved to protect and maintain homeostasis in the lung. Despite these sophisticated defense mechanisms, the respiratory system remains highly susceptible to environmental challenges. Because of the impact of respiratory exposure on human health and disease, there has been considerable interest in developing reliable and predictive in vitro model systems for respiratory toxicology and basic research. Human air-liquid-interface (ALI) organotypic airway tissue models derived from primary tracheobronchial epithelial cells have in vivo–like structure and functions when they are fully differentiated. The presence of the air-facing surface allows conducting in vitro exposures that mimic human respiratory exposures. Exposures can be conducted using particulates, aerosols, gases, vapors generated from volatile and semi-volatile substances, and respiratory pathogens. Toxicity data have been generated using nanomaterials, cigarette smoke, e-cigarette vapors, environmental airborne chemicals, drugs given by inhalation, and respiratory viruses and bacteria. Although toxicity evaluations using human airway ALI models require further standardization and validation, this approach shows promise in supplementing or replacing in vivo animal models for conducting research on respiratory toxicants and pathogens.



中文翻译:

特邀综述:源自原代气管支气管上皮细胞的人体气-液-界面器官型气道组织模型——概述和展望

肺是直接暴露于外部环境的器官。鉴于肺的表面积大且通风广泛,它很容易接触空气传播的物质,例如病原体、过敏原、化学物质和颗粒物。已经进化出高度精细和有效的机制来保护和维持肺的体内平衡。尽管有这些复杂的防御机制,但呼吸系统仍然非常容易受到环境挑战的影响。由于呼吸道暴露对人类健康和疾病的影响,人们对开发用于呼吸道毒理学和基础研究的可靠和可预测的体外模型系统产生了相当大的兴趣。源自原代气管支气管上皮细胞的人体气液界面 (ALI) 器官型气道组织模型在完全分化时具有类似体内的结构和功能。面向空气的表面的存在允许进行模拟人类呼吸暴露的体外暴露。可以使用颗粒物、气溶胶、气体、挥发性和半挥发性物质产生的蒸气以及呼吸道病原体进行暴露。使用纳米材料、香烟烟雾、电子烟蒸气、环境空气传播的化学物质、吸入药物以及呼吸道病毒和细菌产生了毒性数据。尽管使用人气道 ALI 模型进行的毒性评估需要进一步标准化和验证,

更新日期:2020-11-12
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