In this study, we aimed to assess the effects of air-exposure treatment on water relations, longevity, and aquaporin-related gene expression of cut ‘Wild Look’ roses ( Rosa hybrida L.). After harvest, cut rose flowers were placed directly in the tap water (exposed to air for 0 h) or exposed to air for 1, 2, or 3 h and then placed in tap water to recover. We determined the effects of air-exposure time on vase life, water relations, morphological and physiological characteristics, bacterial contamination, and on the expression of aquaporin-related gene of cut flowers. Our results revealed that cut flowers exposed to air for 0 h exhibited a highest postharvest quality. Exposure to air for 3 h significantly decreased the postharvest quality of cut flowers cultivated year-round owing to the early failure of water relations, such as maintaining a positive water balance for a short duration, high transpiration under dark conditions, and bacterial proliferation. Notably, in autumn or winter, exposure to air for 1 and 2 h also decreased vase life of cut roses. Exposure to air for 0 h reduced bacterial population at the basal of cut stem ends, decreased water stress, and maintained leaf chlorophyll fluorescence of cut roses cultivated year-round. Exposure to air for 0 h prevented water stress in cut flowers and increased the transcript levels of Rh - PIP2;1 and Rh - TIP in petals, resulting in maintained cell turgor of petals and improved flower diameter of cut roses. Understanding the relationship between air-exposed time and water relations, longevity, and aquaporin-related gene expression of cut roses may help to develop techniques and distribution systems to improve the vase life of cut roses.

中文翻译：

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空气暴露时间对切花玫瑰水分关系、寿命及水通道蛋白相关基因表达的影响

在这项研究中，我们旨在评估空气暴露处理对切开的“野生玫瑰”玫瑰（Rosa hybrida L.）的水分关系、寿命和水通道蛋白相关基因表达的影响。采收后，将切好的月季花直接放入自来水中（暴露于空气中0小时）或暴露于空气中1、2或3小时，然后放入自来水中恢复。我们确定了空气暴露时间对花瓶寿命、水分关系、形态和生理特征、细菌污染以及切花水通道蛋白相关基因表达的影响。我们的结果表明，切花暴露在空气中 0 小时表现出最高的采后质量。由于水分关系的早期失效，暴露在空气中 3 小时显着降低了全年种植的切花的采后质量，例如在短时间内保持正的水平衡、黑暗条件下的高蒸腾作用以及细菌增殖。值得注意的是，在秋季或冬季，在空气中暴露 1 小时和 2 小时也会缩短切花玫瑰的花瓶寿命。暴露在空气中 0 小时减少了切茎末端基部的细菌数量，减少了水分胁迫，并保持了全年栽培的切花玫瑰的叶绿素荧光。暴露在空气中 0 小时可防止切花中的水分胁迫，并增加花瓣中 Rh - PIP2;1 和 Rh - TIP 的转录水平，从而维持花瓣的细胞膨胀并改善切花的花径。了解暴露在空气中的时间与水的关系、寿命的关系，