当前位置: X-MOL 学术Funct. Integr. Genomics › 论文详情
Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)
Genome-wide diversity analysis of TCP transcription factors revealed cases of selection from wild to cultivated barley
Functional & Integrative Genomics ( IF 2.9 ) Pub Date : 2020-11-09 , DOI: 10.1007/s10142-020-00759-4
Guangqi Gao 1 , Jinhong Kan 1 , Congcong Jiang 1 , Sunny Ahmar 1 , Jing Zhang 1 , Ping Yang 1
Affiliation  

Plant-specific TEOSINTE BRANCHED 1/CYCLOIDEA/PROLIFERATING CELL FACTORS 1/2 (TCP) transcription factors have known roles in inflorescence architecture. In barley, there are two family members INTERMEDIUM-C (INT-c/HvTB1-1) and COMPOSITUM 1 (COM1/HvTCP24) which are involved in the manipulation of spike architecture, whereas the participation of TCP family genes in selection from wild (Hordeum vulgare subsp. spontaneum, Hs) to cultivated barley (Hordeum vulgare subsp. vulgare, Hv) remains poorly investigated. Here, by conducting a genome-wide survey for TCP-like sequences in publicly-released datasets, 22 HsTCP and 20 HvTCP genes encoded for mature proteins were identified and assigned into two classes (I and II) based on their functional domains and the phylogenetic analysis. Each counterpart of the orthologous gene in wild and cultivated barley usually represented a similarity on the transcriptional profile across the tissues. The diversity analysis of TCPs in 90 wild barley accessions and 137 landraces with geographically-referenced passport information revealed the detectable selection at three loci including INT-c/HvTB1-1, HvPCF2, and HvPCF8. Especially, the HvPCF8 haplotypes in cultivated barley were found correlating with their geographical collection sites. There was no difference observed in either transactivation activity in yeast or subcellular localization in Nicotiana benthamiana among these haplotypes. Nevertheless, the genome-wide diversity analysis of barley TCP genes in wild and cultivated populations provided insight for future functional characterization in plant development such as spike architecture.



中文翻译:

TCP 转录因子的全基因组多样性分析揭示了从野生大麦到栽培大麦的选择案例

植物特异性 TEOSINTE BRANCHED 1/CYCLOIDEA/PROLIFERATING CELL FACTORS 1/2 (TCP) 转录因子在花序结构中具有已知作用。在大麦中,有两个家族成员INTERMEDIUM-C ( INT-c / HvTB1-1 ) 和COMPOSITUM 1 ( COM1/HvTCP24 ) 参与了穗结构的操纵,而 TCP 家族基因参与了从野生 ( Hordeum vulgare subsp. spontaneum , Hs ) 到栽培大麦 ( Hordeum vulgare subsp. vulgare , Hv )) 仍然缺乏调查。在这里,通过在公开发布的数据集中对 TCP 样序列进行全基因组调查,鉴定了为成熟蛋白质编码的22 个HsTCP和 20 个HvTCP基因,并根据它们的功能域和系统发育将它们分为两类(I 和 II)。分析。野生和栽培大麦中直向同源基因的每个对应物通常代表组织中转录谱的相似性。具有地理参考护照信息的 90 个野生大麦种质和 137 个地方品种中TCP的多样性分析揭示了三个位点的可检测选择,包括INT-c/HvTB1-1HvPCF2HvPCF8. 特别是,发现栽培大麦中的HvPCF8单倍型与其地理收集地点相关。在这些单倍型中,在酵母中的反式激活活性或本氏烟草中的亚细胞定位方面没有观察到差异。尽管如此,野生和栽培种群中大麦TCP基因的全基因组多样性分析为未来植物发育的功能表征(如穗结构)提供了见解。

更新日期:2020-11-12
down
wechat
bug