Broad bean wilt virus 1 (BBWV-1, genus Fabavirus, family Secoviridae) is a bipartite positive single-stranded RNA (+ssRNA) virus infecting important horticultural and ornamental crops worldwide. RNA1 encodes proteins involved in virus replication, whereas RNA2 encodes the large and small coat proteins (LCP, and SCP, respectively) and two putative movement proteins with overlapping C-terminal but different sizes: 47.2 kDa (VP47) and 37 kDa (VP37). Post-transcriptional gene silencing (PTGS) is a mechanism of gene regulation and defense against pathogens such as viruses. However, most plant viruses encode proteins called viral suppressors of RNA silencing (VSRs) which able to inhibit PTGS. Previously, BBWV-1 VP37 was identified as VSR and here, we have demonstrated that VP47 and SCP, but not LCP, are also VSRs by two approaches: (i) transient expression in Nicotiana benthamiana 16c plants expressing the green fluorescent protein (GFP) constitutively, and (ii) movement complementation assays using a viral vector based on Turnip crinkle virus sequence containing GFP (pTCV-GFP). In contrast LCP but not SCP of Broad been wilt virus 2 (BBWV-2, genus Fabavirus, family Secoviridae) is a VSR, which illustrates that viruses of the same genus can interact differently with the host.

蚕豆萎蔫病毒1（BBWV-1，属Fabavirus，家庭Secoviridae）是一种双向阳性单链RNA（+ ssRNA）病毒，可感染世界各地重要的园艺和观赏作物。RNA1编码参与病毒复制的蛋白质，而RNA2编码大和小外壳蛋白（分别为LCP和SCP）以及两个假定的运动蛋白，其C端重叠但大小不同：47.2 kDa（VP47）和37 kDa（VP37） 。转录后基因沉默（PTGS）是一种基因调节和防御病原体（例如病毒）的机制。但是，大多数植物病毒编码的蛋白被称为RNA沉默病毒抑制蛋白（VSR），能够抑制PTGS。以前，BBWV-1 VP37被识别为VSR，在这里，我们已经通过两种方法证明VP47和SCP而非LCP也是VSR：（i）在本氏烟草中瞬时表达16c植物组成性表达绿色荧光蛋白（GFP），以及（ii）使用基于包含GFP（pTCV-GFP）的芜菁皱纹病毒序列的病毒载体进行运动互补测定。相比之下，LCP而不是广泛传播的枯萎病毒2（BBWV-2，Fabavirus属，Secoviridae家族）的SCP是VSR，这说明同一属的病毒可以与宿主进行不同的相互作用。